Sehrt I, Käppler W, Lange A
Z Erkr Atmungsorgane. 1976;144(2):146-54.
Experiences with the thin-layer chromatography of mycobacterial lipids for the differentiation of the species M. avium and M. intracellulare are described. Details of the procedure used have been published in a recent paper. The 61 strains investigated belong to 16 different serotypes. Twenty of the 21 strains of M. avium present three distinct lipid patterns corresponding to the serotypes 1, 2 and 3. One strain had an aberrant lipid pattern suggesting that serotyping was at fault. The results obtained with 40 M. intracellulare strains of the serotypes III, IV, VI, VII, Davis, Watson, Howell, Chance, Boone, Yandle, Wilson, Altman and Arnold show that a differentiation by thin-layer chromatography of their lipids--using two different developing solvents--is possible. In allbut one case there was a complete correspondence between classification by lipid analysis and the results of serological typing. The distinction between M. avium and M intracellulare on the basis of species specific lipid patterns is not possible. This supports the conclusion that M. avium and M. intracellulare appear to be the same species having a number of distinctive serotypes.
描述了用于区分鸟分枝杆菌和胞内分枝杆菌的分枝杆菌脂质薄层层析的经验。所用方法的详细信息已发表在最近的一篇论文中。所研究的61株菌株属于16种不同的血清型。21株鸟分枝杆菌中的20株呈现出与血清型1、2和3相对应的三种不同的脂质模式。一株菌株具有异常的脂质模式，表明血清分型有误。对血清型III、IV、VI、VII、戴维斯、沃森、豪厄尔、钱斯、布恩、扬德尔、威尔逊、奥特曼和阿诺德的40株胞内分枝杆菌菌株的研究结果表明，使用两种不同的展开溶剂通过脂质薄层层析对它们进行区分是可能的。除一例之外，脂质分析分类与血清学分型结果在所有情况下都完全一致。基于种特异性脂质模式区分鸟分枝杆菌和胞内分枝杆菌是不可能的。这支持了鸟分枝杆菌和胞内分枝杆菌似乎是具有许多独特血清型的同一物种这一结论。
