通过非典型分枝杆菌表面抗原的薄层色谱法进行鉴定。
Identification of atypical mycobacteria by thin-layer chromatography of their surface antigens.
作者信息
Brennan P J, Souhrada M, Ullom B, McClatchy J K, Goren M B
出版信息
J Clin Microbiol. 1978 Oct;8(4):374-9. doi: 10.1128/jcm.8.4.374-379.1978.
Abstract
The knowledge that the surface (Schaefer) antigens of certain smooth-colony atypical mycobacteria are multiglycosylated C-mycosidic peptidoglycolipids was used to devise a sensitive thin-layer chromatographic (TLC) procedure for the identification of Mycobacterium avium/M. intracellulare/M. scrofulaceum serotypes. TLC maps of the type-specific peptidoglycolipids from 17 of the 31 serotypes are presented. The primary use of the technique is to corroborate results obtained by seroagglutination. Without the aid of seroagglutination, the TLC procedure almost invariably requires the availability of reference strains or the specific peptidoglycolipids derived therefrom.
摘要
某些光滑菌落非典型分枝杆菌的表面（谢弗）抗原是多糖基化的C-糖苷肽糖脂，利用这一知识设计了一种灵敏的薄层色谱（TLC）程序，用于鉴定鸟分枝杆菌/胞内分枝杆菌/瘰疬分枝杆菌血清型。给出了31种血清型中17种的型特异性肽糖脂的TLC图谱。该技术的主要用途是确证血清凝集试验所得结果。若无血清凝集试验辅助，TLC程序几乎总是需要有参考菌株或从中提取的特异性肽糖脂。
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本文引用的文献
1
THE PATHOGENIC "ATYPICAL" MYCOBACTERIA.致病性“非典型”分枝杆菌
Annu Rev Microbiol. 1963;17:473-94. doi: 10.1146/annurev.mi.17.100163.002353.
2
Serologic identification of the atypical mycobacteria and its value in epidemiologic studies.
Am Rev Respir Dis. 1967 Jul;96(1):115-8. doi: 10.1164/arrd.1967.96.1.115.
3
Serologic identification and classification of the atypical mycobacteria by their agglutination.通过凝集反应对非典型分枝杆菌进行血清学鉴定和分类。
Am Rev Respir Dis. 1965 Dec;92(6):85-93. doi: 10.1164/arrd.1965.92.6P2.85.
4
[Structure of a peptidolipid glycoside isolated from a Mycobacterium].[从分枝杆菌中分离出的一种肽脂糖苷的结构]
Eur J Biochem. 1968 Sep 24;5(4):487-91. doi: 10.1111/j.1432-1033.1968.tb00396.x.
5
[Studies on the mycoside C 2 of the Mycobacterium avium. Structure determination].[鸟分枝杆菌的霉菌糖苷C2研究。结构测定]
Eur J Biochem. 1971 Jul 29;21(2):285-91. doi: 10.1111/j.1432-1033.1971.tb01468.x.
6
Thin-layer chromatography of mycobacterial lipids as an aid to classification: technical improvements: mycobacterium avium, M. intracellulare (Battey bacilli).
Tubercle. 1971 Sep;52(3):219-25. doi: 10.1016/0041-3879(71)90044-4.
7
Thin-layer chromatography of mycobacterial lipids as an aid to classification: the scotochromogenic mycobacteria, including Mycobacterium scrofulaceum, M. xenopi, M. aquae, M. gordonae, M. flavescens.分枝杆菌脂质的薄层色谱法在分类中的应用：暗产色分枝杆菌，包括瘰疬分枝杆菌、偶发分枝杆菌、水分枝杆菌、戈登分枝杆菌、微黄分枝杆菌。
Tubercle. 1972 Jun;53(2):118-27. doi: 10.1016/0041-3879(72)90028-1.
8
Pathogenic mycobacteria.
Bibl Tuberc. 1965;21:235-87.
9
Serological examination of some strains that are in the Mycobacterium avium-intracellulare-scrofulaceum complex but do not belong to Schaefer's serotypes.对鸟分枝杆菌-胞内分枝杆菌-瘰疬分枝杆菌复合群中一些不属于谢弗血清型的菌株进行血清学检查。
Appl Microbiol. 1973 Oct;26(4):470-3. doi: 10.1128/am.26.4.470-473.1973.
10
Mycobacterial lipids: selected topics.分枝杆菌脂质：选定主题。
Bacteriol Rev. 1972 Mar;36(1):33-64. doi: 10.1128/br.36.1.33-64.1972.
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