Amanfu W, Masupu K V, Adom E K, Raborokgwe M V, Bashiruddin J B
National Veterinary Laboratory, Gaborone, Botswana.
Vet Rec. 1998 Jul 11;143(2):46-8. doi: 10.1136/vr.143.2.46.
An outbreak of contagious bovine pleuropneumonia (CBPP) was detected in Botswana in 1995 after more than half a century of freedom from the disease. Lung tissues, pleural fluids, nasal swabs and serum samples were examined in laboratories in Botswana, South Africa and Namibia and the findings were confirmed in Italy. The disease was confirmed as CBPP from the gross and histopathological changes in the lungs of affected animals and by the culture of the agent of CBPP, Mycoplasma mycoides subspecies mycoides, small colony variant (MmmSC). These findings were supported by the demonstration of specific complement-fixing antibodies and the production of polymerase chain reaction products of MmmSC.
1995年，在博茨瓦纳已半个多世纪未出现传染性牛胸膜肺炎（CBPP）疫情后，该国再次检测到该疫情。博茨瓦纳、南非和纳米比亚的实验室对肺组织、胸水、鼻拭子和血清样本进行了检测，检测结果在意大利得到确认。根据患病动物肺部的大体和组织病理学变化以及对CBPP病原体——丝状支原体丝状亚种小菌落变种（MmmSC）的培养，确诊该病为CBPP。特异性补体结合抗体的检测以及MmmSC聚合酶链反应产物的产生也支持了这些检测结果。
