儿童自然感染G1和G4型轮状病毒后通过免疫印迹法检测的血清学反应。

Serologic responses by immunoblot following natural infection with rotavirus serotypes G1 and G4 in children.

作者信息

Begue R E, Martin P, Dennehy P H

机构信息

Department of Pediatrics, Brown University School of Medicine, Providence, Rhode Island, USA.

出版信息

J Med Virol. 1998 Sep;56(1):52-7. doi: 10.1002/(sici)1096-9071(199809)56:1<52::aid-jmv9>3.0.co;2-m.

DOI:10.1002/(sici)1096-9071(199809)56:1<52::aid-jmv9>3.0.co;2-m

PMID:9700633

Abstract

Serologic responses to proteins of rotavirus serotypes G1, P1A[8]; G2, P1B[4]; G3, P1A[8]; and G4, P2A[6] were evaluated by immunoblotting paired sera from 17 children with primary rotavirus infection. Ten children were infected with G1, P1A[8]; five with G4, P1A[8]; and two with G4, P2A[6] viruses. Anti-VP6 and anti-VP2 were seen in most responses. Homotypic anti-VP7 developed following G1 and G4 infections in 8 (80%) and 6 (86%) cases, respectively. Homotypic anti-VP4 developed in 9 (60%) cases following P1A[8] infection and in 0 of 2 cases following P2A[6] infection. Heterotypic anti-VP7 appeared against G4 (20%) and G3 (20%) following the 10 G1 infections, and against G3 (86%) and G1 (57%) following the 7 G4 infections. Heterotypic anti-VP4 occurred in only 3 (18%) children. The data show the antigenic predominance of internal proteins VP6 and VP2. Homotypic antibodies developed against VP7 but not against VP4 in most cases, while heterotypic antibodies were infrequent.

摘要

通过免疫印迹法对17例原发性轮状病毒感染儿童的配对血清进行检测，评估其对轮状病毒血清型G1、P1A[8]；G2、P1B[4]；G3、P1A[8]；以及G4、P2A[6]蛋白质的血清学反应。10名儿童感染了G1、P1A[8]病毒；5名儿童感染了G4、P1A[8]病毒；2名儿童感染了G4、P2A[6]病毒。大多数反应中可检测到抗VP6和抗VP2抗体。G1和G4感染后，分别有8例（80%）和6例（86%）出现同型抗VP7抗体。P1A[8]感染后，9例（60%）出现同型抗VP4抗体，P2A[6]感染的2例中均未出现。10例G1感染后，出现针对G4（20%）和G3（20%）的异型抗VP7抗体；7例G4感染后，出现针对G3（86%）和G1（57%）的异型抗VP7抗体。仅3例（18%）儿童出现异型抗VP4抗体。数据显示内部蛋白VP6和VP2具有抗原优势。大多数情况下，针对VP7产生了同型抗体，而针对VP4未产生同型抗体，异型抗体则很少见。