Richardson S C, Bishop R F
Department of Gastroenterology, Royal Children's Hospital, Parkville, Victoria, Australia.
J Clin Microbiol. 1990 Sep;28(9):1891-7. doi: 10.1128/jcm.28.9.1891-1897.1990.
The class-specific antibody responses to serotype 1 rotavirus structural proteins were examined by immunoblotting with sera obtained from young children hospitalized with acute rotavirus diarrhea caused by serotype 1. All were believed to be primary infections. Three consecutive samples were obtained from 16 patients during the acute and convalescent phases of the disease and then approximately 4 months later. Immunoglobulin G (IgG)-class antibody responses to two inner capsid proteins (VP2 and VP6) and to the major homologous outer capsid protein (VP7) were detected in all patients. Antibody responses to VP6 were rapid, increased in intensity during 20 to 40 days after the onset of symptoms, and persisted for more than 4 months. Responses to VP2 and VP7 were more delayed, were maximal in convalescent-phase sera, and decreased markedly in intensity 4 months after the onset of symptoms in the majority of children. Two patients with evidence of mixed infection showed persisting high levels of antibody to VP7. Responses to the outer capsid protein VP4 were detected in 67% of patients, peaked at 20 to 40 days after the onset of symptoms, and were no longer detected at 4 months in the majority of patients. It is likely that the immunoblotting technique underestimated responses to VP4. Acute- and convalescent-phase sera (known to contain antirotavirus IgM or IgA measured by enzyme immunoassay) were also examined by immunoblotting. IgM- and IgA-class antibody responses to viral proteins VP2, VP4, and VP7 appeared to be qualitatively identical to those observed for IgG in the same serum samples.
采用免疫印迹法，以1型血清型引起的急性轮状病毒腹泻住院幼儿的血清，检测针对1型血清型轮状病毒结构蛋白的类别特异性抗体反应。所有病例均被认为是初次感染。在疾病的急性期和恢复期以及大约4个月后，从16名患者身上采集了连续3份样本。在所有患者中均检测到针对两种内衣壳蛋白（VP2和VP6）以及主要同源外衣壳蛋白（VP7）的免疫球蛋白G（IgG）类别抗体反应。对VP6的抗体反应迅速，在症状出现后20至40天内强度增加，并持续超过4个月。对VP2和VP7的反应出现较迟，在恢复期血清中达到峰值，大多数儿童在症状出现4个月后强度明显下降。两名有混合感染证据的患者显示VP7抗体水平持续较高。67%的患者检测到对外衣壳蛋白VP4的反应，在症状出现后20至40天达到峰值，大多数患者在4个月时不再检测到。免疫印迹技术可能低估了对VP4的反应。还通过免疫印迹法检测了急性期和恢复期血清（已知通过酶免疫测定法含有抗轮状病毒IgM或IgA）。针对病毒蛋白VP2、VP4和VP7的IgM和IgA类别抗体反应在质量上似乎与同一血清样本中观察到的IgG反应相同。
