Silz S, Valet G, Tongendorff J, Strecker W, Ruhenstroth-Bauer G
Acta Hepatogastroenterol (Stuttg). 1976 Jul-Aug;23(4):255-61.
One time i.p. injections of 5 -20 mg Pregnenolone-16alpha-carbonitrile (PCN) effects the mitotic activity of rat liver cells during a time period of 24 hours to be 20 times higher than the normal mitotic rate. This, however, does not result in an measurable increase of cell numbers and cell volumes. Furthermore, total liver DNA, RNA, protein and dry-weight remain unchanged. The injection of 7 times 10 mg PCN also results in the elevation of the mitotic rate. Return of the elevated mitotic rate to base level takes 18 days from the time the last injection of PCN was administered. During this time the number and the average volume of liver cells increases by 28% and 30%, respectively. The dry weight of liver and tetraploid nuclei increase in number, whereas RNA- and protein content remains unchanged. All parameters have reached the base line after 4 - 6 weeks following the last injection of PCN. No histological changes were observed after multiple doses of PCN that cause hyperplasia and hypertrophy of liver tissue. Hypertrophy of liver cells follows the observed hyperplasia and therefore is not expected to be the cause of the hyperplastic processes.
一次性腹腔注射5 - 20毫克孕烯醇酮-16α-腈（PCN），在24小时内可使大鼠肝细胞的有丝分裂活性比正常有丝分裂速率高20倍。然而，这并未导致细胞数量和细胞体积出现可测量的增加。此外，肝脏总DNA、RNA、蛋白质和干重保持不变。注射7次10毫克PCN也会导致有丝分裂速率升高。从最后一次注射PCN开始，有丝分裂速率升高后恢复到基线水平需要18天。在此期间，肝细胞数量和平均体积分别增加28%和30%。肝脏干重和四倍体细胞核数量增加，而RNA和蛋白质含量保持不变。在最后一次注射PCN后的4 - 6周，所有参数均达到基线。多次注射PCN导致肝组织增生和肥大后，未观察到组织学变化。肝细胞肥大发生在观察到的增生之后，因此预计不是增生过程的原因。
