高速逆流色谱法从红腺忍冬全草中分离纯化冬凌草甲素。
Isolation and purification of oridonin from the whole plant of Isodon rubescens by high-speed counter-current chromatography.
机构信息
College of Pharmacy, Harbin University, Daqing 163319, China.
出版信息
Molecules. 2011 Sep 14;16(9):7949-57. doi: 10.3390/molecules16097949.
Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, 1H-NMR and 13C-NMR.
半制备高速逆流色谱（HSCCC）成功地用于分离和纯化由冬凌草组成的冬凌草甲素，使用两相溶剂系统由正己烷-乙酸乙酯-甲醇-水（2.8:5:2.8:5，v/v/v/v）。通过 HSCCC 分离、收集和纯化的目标化合物通过高效液相色谱（HPLC）进行分析。从 100 毫克粗冬凌草提取物中，在不到 100 分钟内获得了 40.6 毫克纯度为 73.5%的冬凌草甲素。该化合物的化学结构通过 IR、1H-NMR 和 13C-NMR 进行鉴定。
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