Boda D, Németh I, Pintér S
Department of Pediatrics, Albert Szent-Györgyi Medical University, Szeged, Hungary.
Biol Neonate. 1998 Oct;74(4):281-8. doi: 10.1159/000014035.
Determination of the surface tension (ST), the total glutathione (GL) content and the ratio of oxidized glutathione (GSSG) to reduced glutathione (GSH) in the tracheal aspirate (TA) of newborn infants with IRDS.
The ST of the TA was determined by monitoring the fluid level pulsated in a capillary glass tube by means of a digitalized videocomputerized picture analysis program, a technique developed in our laboratory. The concentrations of GSSG and total GL in the TA were determined enzymatically with glutathione reductase. All results of laboratory tests were referred to the total phospholipid (PL) concentration. Patients, Experimental Material: TA samples were collected from 32 intubated premature and newborn infants admitted to the NICU with IRDS during the first 2 weeks of their lives. Control samples were obtained from 11 children prior to elective surgery.
The ST relative to the PL content (surface tension index, STI) was significantly lower in the newborns with IRDS than in the control group, and the concentration of GSH in the TA was also markedly decreased in all IRDS infants studied. The concentration of GSSG and the ratio of GSSG to GSH were significantly higher in the severe cases and in those with an unfavourable prognosis. Surfactant treatment had a protective effect against oxidative stress, it induced a decrease in both the GSSG concentration and in the GL redox ratio (GSSG/GSH) in the TA. There was a close correlation between the GSH content and the STI value of the samples studied.
Oxidation and consequent depletion of GSH in the TA may be an aggravating factor in the development of the insufficient surface activity in intubated newborns with IRDS.
测定患有呼吸窘迫综合征（IRDS）的新生儿气管吸出物（TA）的表面张力（ST）、总谷胱甘肽（GL）含量以及氧化型谷胱甘肽（GSSG）与还原型谷胱甘肽（GSH）的比例。
TA的ST通过我们实验室开发的一种技术，即利用数字化视频计算机化图像分析程序监测毛细管玻璃管中脉动的液位来测定。TA中GSSG和总GL的浓度用谷胱甘肽还原酶酶法测定。所有实验室检测结果均以总磷脂（PL）浓度为参照。患者、实验材料：从32例入住新生儿重症监护病房（NICU）且患有IRDS的插管早产和新生儿在其出生后的前2周内采集TA样本。对照样本取自11名择期手术前的儿童。
患有IRDS的新生儿中，相对于PL含量的ST（表面张力指数，STI）显著低于对照组，并且在所有研究的IRDS婴儿中，TA中GSH的浓度也明显降低。在重症病例和预后不良的病例中，GSSG的浓度以及GSSG与GSH的比例显著更高。表面活性剂治疗对氧化应激具有保护作用，它导致TA中GSSG浓度和GL氧化还原比（GSSG/GSH）均降低。所研究样本的GSH含量与STI值之间存在密切相关性。
TA中GSH的氧化及随之而来的消耗可能是患有IRDS的插管新生儿表面活性不足发展过程中的一个加重因素。
