Chandler L J, Blair C D, Beaty B J
Arthropod-Borne and Infectious Diseases Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
J Med Entomol. 1998 Jul;35(4):567-72. doi: 10.1093/jmedent/35.4.567.
La Crosse (LAC) virus is efficiently transmitted transovarially by the mosquito Aedes triseriatus (Say). To determine the time course and tropisms of LAC virus infection of ovaries, immunofluorescent antibody staining, in situ hybridization, and reverse transcription-polymerase chain reaction techniques were used to detect viral antigen and RNA in the ovaries. LAC virus was detected in the ovaries (presumably in calyx tissues) by all 3 assays at day 2 after infection and before dissemination from the midgut on day 6. Apparently, ovaries can become infected by mechanisms other than by dissemination of virus from a midgut infection. By days 8-14 after infection, virus analytes became detectable in many tissues within the ovary including follicular epithelium, oocytes, nurse cells, and calyx, reflecting the remarkable host parasite relationship between LAC virus and its mosquito vector.
拉克罗斯（LAC）病毒可通过三带喙库蚊（Say）高效经卵传播。为确定卵巢感染LAC病毒的时间进程和嗜性，采用免疫荧光抗体染色、原位杂交和逆转录聚合酶链反应技术检测卵巢中的病毒抗原和RNA。感染后第2天，即在病毒于第6天从中肠扩散之前，通过所有这三种检测方法均在卵巢（可能在萼组织中）检测到了LAC病毒。显然，卵巢可通过病毒从中肠感染扩散以外的机制被感染。感染后第8 - 14天，在卵巢内的许多组织中均可检测到病毒分析物，包括卵泡上皮、卵母细胞、滋养细胞和萼，这反映了LAC病毒与其蚊媒之间显著的宿主 - 寄生虫关系。
