Borucki Monica K, Chandler Laura J, Parker Beulah M, Blair Carol D, Beaty Barry J
Arthropod-borne and Infectious Diseases Laboratory (AIDL), Department of Microbiology, Colorado State University, Fort Collins, CO 80523-1682, USA1.
Department of Entomology, North Carolina State University, Raleigh, NC 27965, USA2.
J Gen Virol. 1999 Dec;80 ( Pt 12):3173-3179. doi: 10.1099/0022-1317-80-12-3173.
Rapid evolution of bunyaviruses may occur by RNA segment reassortment between closely related viruses. Reassortment between viruses occurs in dually infected mosquitoes when two different viruses are simultaneously ingested or when the second virus is ingested within 2 days of the first virus. By 3 days after oral infection, interference to superinfection occurs, thus limiting the potential for evolution. Aedes triseriatus mosquitoes can also be transovarially infected (TI+) with LaCrosse (LAC) virus. In these studies, the potential for oral superinfection of TI+ mosquitoes was assessed. Approximately 20% of mosquitoes TI+ with either a temperature-sensitive LAC virus or wild-type (wt) LAC virus became superinfected by ingesting blood meals containing wt LAC or snowshoe hare (SSH) viruses. LAC virus TI+ mosquitoes superinfected with SSH virus were detected by blot hybridization or RT-PCR. Viruses from these mosquitoes were plaque purified and genotyped using RT-PCR. Reassortant genomes were detected in 2.3% of the viruses genotyped, and 4.0% of the genomes tested were diploid for one genome segment.
布尼亚病毒可能通过密切相关病毒之间的RNA片段重配而快速进化。当两种不同病毒同时被摄入或第二种病毒在第一种病毒摄入后2天内被摄入时，病毒之间的重配发生在双重感染的蚊子中。口服感染后3天，会出现对超感染的干扰，从而限制了进化的可能性。三带喙库蚊也可经卵感染（TI+）拉科罗斯（LAC）病毒。在这些研究中，评估了TI+蚊子口服超感染的可能性。大约20%经温度敏感型LAC病毒或野生型（wt）LAC病毒TI+的蚊子通过摄取含有wt LAC或雪兔（SSH）病毒的血餐而发生超感染。通过印迹杂交或RT-PCR检测到被SSH病毒超感染的LAC病毒TI+蚊子。对这些蚊子的病毒进行空斑纯化，并使用RT-PCR进行基因分型。在2.3%的基因分型病毒中检测到重配基因组，并且在测试的基因组中有4.0%的一个基因组片段是二倍体。
