Köhidai L, Csaba G
Department of Genetics, Cell- and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary.
Cytokine. 1998 Jul;10(7):481-6. doi: 10.1006/cyto.1997.0328.
Three representative cytokines interleukin (IL-8), RANTES and tumour necrosis factor alpha (TNF-alpha) have a concentration-dependent chemotactic effect on the unicellular Tetrahymena. Maximal effective concentrations of IL-8 (1 ng/ml) and RANTES (75 ng/ml) are in the same range as in mammals, which indicates an evolutionary background of physiological effects elicited. Progeny generations of cells selected for their affinity to cytokines (IL-8 and TNF-alpha) show an enhanced positive chemosensory reaction to the cytokines. The changed reaction of these cells to the chemoattraction of the culturing medium was also observed. The results call attention to the presence of cytokine-dependent processes at a low phylogenetic level.
三种具有代表性的细胞因子，即白细胞介素-8（IL-8）、调节活化正常T细胞表达和分泌的趋化因子（RANTES）以及肿瘤坏死因子-α（TNF-α），对单细胞草履虫具有浓度依赖性趋化作用。IL-8（1 ng/ml）和RANTES（75 ng/ml）的最大有效浓度与哺乳动物中的浓度范围相同，这表明所引发的生理效应具有进化背景。因对细胞因子（IL-8和TNF-α）具有亲和力而被选择的细胞子代，对这些细胞因子表现出增强的正向化学感应反应。还观察到这些细胞对培养基化学吸引的反应发生了变化。这些结果提醒人们注意在低系统发育水平上存在细胞因子依赖性过程。
