乳糜泻中的自身抗体：成纤维细胞的重要性。

Autoantibodies in celiac disease: importance of fibroblasts.

作者信息

Sulkanen S, Halttunen T, Marttinen A, Leivo E L, Laurila K, Mäki M

机构信息

Institute of Medical Technology, University of Tampere, Finland.

出版信息

J Pediatr Gastroenterol Nutr. 1998 Aug;27(2):206-13. doi: 10.1097/00005176-199808000-00015.

DOI:10.1097/00005176-199808000-00015

PMID:9702655

Abstract

BACKGROUND

Serum reticulin and endomysium autoantibodies are highly celiac disease-specific, and the autoantigens have been shown to be derived from human fibroblasts. Among human tissues, the umbilical cord also expresses these antigens. This study was conducted to compare different autoantibody tests and especially to elucidate whether human umbilical cord is a suitable substrate in tests and whether the cord jelly-derived fibroblasts express the antigens.

METHODS

The indirect immunofluorescence method was used to detect the tissue and Wharton's jelly-derived fibroblast antibodies in 334 celiac disease and control sera samples. Affinity chromatography studies were used to show the correlation between human fibroblast-derived autoantigens and tissue and gliadin antibodies. The jelly-derived fibroblasts were used as antigen in a whole-cell enzyme-linked immunosorbent assay.

RESULTS

Celiac disease patient sera showed IgA-class human umbilical cord antibody with high sensitivity (100%) and specificity (99%). All celiac disease patient sera tested showed in indirect immunofluorescence the molecules expressed by Wharton's jelly-derived fibroblasts. The whole-cell fibroblast autoantibody enzyme-linked immunosorbent assay had a sensitivity of 100% and a specificity of 81%. Human fibroblast-derived celiac disease autoantigens absorbed most of the IgA responsible for human umbilical cord antibodies but not the IgA responsible for gliadin antibodies in the same sera.

CONCLUSIONS

Wharton's jelly-derived fibroblast autoantibodies tested in a novel whole-cell enzyme-linked immunosorbent assay correlated well with the human umbilical cord but not with gliadin antibodies.

摘要

背景

血清网硬蛋白和肌内膜自身抗体对乳糜泻具有高度特异性，且已证明自身抗原来源于人成纤维细胞。在人体组织中，脐带也表达这些抗原。本研究旨在比较不同的自身抗体检测方法，尤其是阐明人脐带在检测中是否是合适的底物，以及脐带胶衍生的成纤维细胞是否表达这些抗原。

方法

采用间接免疫荧光法检测334份乳糜泻患者血清和对照血清样本中的组织及华通氏胶衍生的成纤维细胞抗体。亲和层析研究用于显示人成纤维细胞衍生的自身抗原与组织及麦醇溶蛋白抗体之间的相关性。将胶衍生的成纤维细胞用作全细胞酶联免疫吸附测定中的抗原。

结果

乳糜泻患者血清显示出IgA类人脐带抗体，具有高敏感性（100%）和特异性（99%）。所有检测的乳糜泻患者血清在间接免疫荧光中均显示华通氏胶衍生的成纤维细胞表达的分子。全细胞成纤维细胞自身抗体酶联免疫吸附测定的敏感性为100%，特异性为81%。人成纤维细胞衍生的乳糜泻自身抗原吸收了同一血清中大部分负责与人脐带抗体结合的IgA，但未吸收负责与麦醇溶蛋白抗体结合的IgA。