Korponay-Szabó I R, Sulkanen S, Halttunen T, Maurano F, Rossi M, Mazzarella G, Laurila K, Troncone R, Mäki M
Institute of Medical Technology, Department of Pediatrics, University of Tampere, Finland.
J Pediatr Gastroenterol Nutr. 2000 Nov;31(5):520-7. doi: 10.1097/00005176-200011000-00013.
Endomysial antibodies have recently been shown to react with tissue transglutaminase. This study was undertaken to investigate whether the tissue distribution of transglutaminase is also compatible with reticulin, jejunal, and fibroblast autoantibody binding patterns.
Sera from patients with and without celiac disease, monoclonal tissue transglutaminase antibodies, and sera from mice parenterally immunized against commercially available tissue transglutaminase, transglutaminase complexed with gliadin, or gliadin were used in indirect immunofluorescence and double-staining studies using both rodent and primate tissues as substrates. Also, antibody competition, affinity chromatography, and potassium thiocyanate extraction studies were undertaken.
Tissue transglutaminase antibody binding patterns were identical with the extracellular binding patterns seen with celiac patient sera. Human umbilical cord-derived fibroblasts exhibited both cytoplasmic and extracellular matrix staining. Double staining with patients' sera and tissue transglutaminase antibodies showed complete overlapping. Tissue transglutaminase effectively absorbed reticulin-endomysial antibodies from celiac sera, and patients' sera blocked the staining of the monoclonal tissue transglutaminase antibodies. Potassium thiocyanate extraction abolished the staining patterns, but they were elicited again after readdition of tissue transglutaminase.
Reticulin, endomysial, and jejunal antibodies detect transglutaminase in both rodent and primate tissues, indicating that these tissue autoantibodies are identical.
近来研究显示肌内膜抗体可与组织转谷氨酰胺酶发生反应。本研究旨在探讨转谷氨酰胺酶的组织分布是否也与网硬蛋白、空肠及成纤维细胞自身抗体的结合模式相符。
采用腹腔疾病患者和非患者的血清、单克隆组织转谷氨酰胺酶抗体，以及经皮下注射免疫了市售组织转谷氨酰胺酶、与麦醇溶蛋白复合的转谷氨酰胺酶或麦醇溶蛋白的小鼠血清，以啮齿动物和灵长类动物组织为底物进行间接免疫荧光和双重染色研究。此外，还进行了抗体竞争、亲和层析及硫氰酸钾提取研究。
组织转谷氨酰胺酶抗体的结合模式与腹腔疾病患者血清的细胞外结合模式相同。人脐带来源的成纤维细胞呈现出细胞质和细胞外基质染色。患者血清与组织转谷氨酰胺酶抗体的双重染色显示完全重叠。组织转谷氨酰胺酶可有效从腹腔疾病患者血清中吸收网硬蛋白 - 肌内膜抗体，且患者血清可阻断单克隆组织转谷氨酰胺酶抗体的染色。硫氰酸钾提取消除了染色模式，但在重新添加组织转谷氨酰胺酶后又再次出现。
网硬蛋白、肌内膜和空肠抗体可在啮齿动物和灵长类动物组织中检测到转谷氨酰胺酶，表明这些组织自身抗体是相同的。
