Krivorutchenko Iu L
Vopr Virusol. 1998 May-Jun;43(3):122-4.
The effects of a cation detergent myramistin on free infectious HIV-1 LAI strain are studied. HIV-containing culture fluid was incubated with myramistin, after which Jurkat-tat T-cells were infected with this fluid in various dilutions. The number of viable cells was counted and production of HIV p24 assessed on days 3, 7, 11, and 14 of experiment. Myramistin in a concentration of 100 micrograms/ml prevented the replication of HIV-1 in indicator cells. Addition of 0.50 microgram/ml of myramistin to culture fluid led to 1000-fold drop of virus infectivity. These results are in line with previous reports about dose-dependent inhibition of cell-associated HIV with the above detergent.
研究了阳离子洗涤剂米拉美斯汀对游离感染性HIV-1 LAI毒株的影响。将含HIV的培养液与米拉美斯汀一起孵育，之后用不同稀释度的该培养液感染Jurkat-tat T细胞。在实验的第3、7、11和14天对活细胞数量进行计数并评估HIV p24的产生情况。浓度为100微克/毫升的米拉美斯汀可阻止HIV-1在指示细胞中的复制。向培养液中添加0.50微克/毫升的米拉美斯汀会导致病毒感染性下降1000倍。这些结果与之前关于上述洗涤剂对细胞相关HIV的剂量依赖性抑制的报道一致。
