Rolf B, Horst B, Eigel A, Sagansermsri T, Brinkmann B, Horst J
Institut für Rechtsmedizin, Westfälische Wilhelms-Universität, Münster, Germany.
Hum Genet. 1998 Jun;102(6):647-52. doi: 10.1007/s004390050757.
Allelic frequencies for up to five short tandem repeat systems (HumTH01. HumVWA, HumF13B, HumCD4, HumD2111) were analyzed in seven population samples from Asia using the polymerase chain reaction and gel electrophoresis. No deviations from Hardy-Weinberg equilibrium were observed. Two new alleles of the CD4 and TH01 loci were detected, and sequenced and their molecular structure is presented. A phylogenetic tree based on Thai, Han Chinese (from the northeast of China), Japanese, German and Ovambo allelic frequencies was constructed and demonstrates the close relationship of the Asian populations. Additionally, allelic frequency data for the VWA and TH01 systems were determined for the south Chinese minorities Bai, Dai and Qiang and for Koreans and compared with the above data. The Bai and Dai populations were clear outliers of the cluster of all other Asians, indicating an unexpected pattern of genetic heterogeneity of the Chinese nation. Two clusters of Asian populations could be established: the Koreans and Japanese together with the Han and Qiang Chinese, and, forming a separate cluster, the Bai and Dai populations.
利用聚合酶链反应和凝胶电泳技术，对来自亚洲的七个群体样本中多达五个短串联重复序列系统（HumTH01、HumVWA、HumF13B、HumCD4、HumD2111）的等位基因频率进行了分析。未观察到偏离哈迪-温伯格平衡的情况。检测到了CD4和TH01基因座的两个新等位基因，并进行了测序，还展示了它们的分子结构。构建了基于泰国人、中国汉族（来自中国东北部）、日本人、德国人及奥万博人等位基因频率的系统发育树，该树表明了亚洲各群体之间的密切关系。此外，还测定了中国南方少数民族白族、傣族和羌族以及韩国人的VWA和TH01系统的等位基因频率数据，并与上述数据进行了比较。白族和傣族群体是所有其他亚洲人群体聚类中的明显异常值，这表明中华民族存在意想不到的遗传异质性模式。可以将亚洲人群体分为两个聚类：韩国人和日本人与汉族和羌族中国人聚在一起，而白族和傣族群体则形成一个单独的聚类。
