Yum S, Takahashi T, Hatta M, Fujisawa T
Department of Developmental Genetics, National Institute of Genetics, Mishima, Japan.
FEBS Lett. 1998 Nov 13;439(1-2):31-4. doi: 10.1016/s0014-5793(98)01314-3.
Hym-176 (APFIFPGPKVamide) is a novel myoactive neuropeptide which was identified in systematic screening of signaling peptides in Hydra magnipapillata. By using PCR and library screening, we cloned and sequenced a full length cDNA which encoded a preprohormone of Hym-176. In the preprohormone, a typical signal sequence, one copy of Hym-176 precursor peptide and one copy of precursor sequence of another novel peptide, Hym-357 (KPAFLFKGYKPamide), were detected. In situ hybridization analysis revealed a strong signal in peduncle neurons. Signals were also detected, though weaker, in neurons in the gastric region and around the mouth. No signals were detected in the two extremities of the body, tentacles and basal disk. The expression pattern is correlated with the distribution of Hym-176 and its myoactive function.
Hym-176（APFIFPGPKVamide）是一种新型的肌活性神经肽，它是在对大型乳头水螅的信号肽进行系统筛选时被鉴定出来的。通过使用聚合酶链反应（PCR）和文库筛选，我们克隆并测序了一个全长cDNA，该cDNA编码Hym-176的前激素原。在前激素原中，检测到一个典型的信号序列、一份Hym-176前体肽以及另一种新型肽Hym-357（KPAFLFKGYKPamide）的一份前体序列。原位杂交分析显示在柄部神经元中有强烈信号。在胃区和口周围的神经元中也检测到了信号，不过较弱。在身体的两端、触手和基盘未检测到信号。这种表达模式与Hym-176的分布及其肌活性功能相关。
