Mitsumoto Y, Watanabe A, Mori A, Koga N
Tokushima New Drug Research Institute, Otsuka Pharmaceutical Co., Ltd., Japan.
Biochem Biophys Res Commun. 1998 Jul 30;248(3):660-3. doi: 10.1006/bbrc.1998.8986.
The spontaneous recovery of nigrostriatal dopaminergic neurons was quantitatively analyzed with tyrosine hydroxylase (TH)-immunocytochemistry in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated C57BL/6 young mice. A substantial reduction of striatal dopamine (DA) level was observed until 24 days following MPTP treatment. The TH-immunoreactive (IR) fibers and number of TH-positive cell bodies were also markedly reduced at 3 days after the toxin treatment. Thereafter, TH-IR fiber densities showed to progressively recover through the examining period. The number of TH-positive cell bodies in substantia nigra pars compacta were not changed during the recovery period. These results indicate that MPTP-treated mice have a potential for spontaneous regenerative sprouting in nigrostriatal dopaminergic system.
利用酪氨酸羟化酶（TH）免疫细胞化学方法，对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）处理的C57BL/6幼鼠黑质纹状体多巴胺能神经元的自发恢复情况进行了定量分析。在MPTP处理后24天内，观察到纹状体多巴胺（DA）水平显著降低。毒素处理3天后，TH免疫反应性（IR）纤维和TH阳性细胞体数量也明显减少。此后，在整个观察期内，TH-IR纤维密度逐渐恢复。黑质致密部TH阳性细胞体数量在恢复期没有变化。这些结果表明，MPTP处理的小鼠黑质纹状体多巴胺能系统具有自发再生芽生的潜力。
