Wahl A, Azaroual N, Imbenotte M, Mathieu D, Forzy G, Cartigny B, Vermeersch G, Lhermitte M
Laboratoire de Biochimie et de Biologie Moléculaire, Hôpital Calmette, Faculté des Sciences Pharmaceutiques et Biologiques, Lille, France.
Toxicology. 1998 Jun 26;128(1):73-81. doi: 10.1016/s0300-483x(98)00055-9.
Analysis of serum and urine samples from three patients in a metabolic acidotic state was performed using biochemical methods and proton nuclear magnetic resonance (1H NMR). Methanol and ethylene glycol were identified by their singlet peaks in the 1H NMR spectra recorded directly from 0.5 ml of the collected samples. Quantification of these compounds was obtained simultaneously with that of their metabolites, formate and glycolate respectively and of lactate and ethanol, the latter being used as an antidote. The NMR results were found to be very similar to the biochemical findings. The results presented here suggest that 1H NMR can be clinically useful since it quickly provides information on the onset of metabolic acidosis and on the biotransformation of xenobiotics.
采用生化方法和质子核磁共振（1H NMR）对三名处于代谢性酸中毒状态患者的血清和尿液样本进行了分析。通过直接从0.5 ml采集样本记录的1H NMR谱中的单峰鉴定出甲醇和乙二醇。这些化合物的定量分析与其代谢物（分别为甲酸和乙醇酸）以及乳酸和乙醇（后者用作解毒剂）的定量分析同时进行。发现NMR结果与生化结果非常相似。此处呈现的结果表明，1H NMR在临床上可能有用，因为它能快速提供有关代谢性酸中毒发作以及外源性物质生物转化的信息。
