Lim V I, Aglyamova G V
Institute of Protein Research, Russian Academy of Sciences, Moscow Region.
Biol Chem. 1998 Jul;379(7):773-81.
Analysis of the available data demonstrated that a codon in the i(th) codon-anticodon duplex should interact with the wobble pair of the i - 1(th) duplex. This interduplex interaction should take place throughout the ribosomal elongation cycle in order to prevent unprogrammed frameshifting. An experimentally observed flexibility of tRNA allows to conserve the interduplex interaction at different mutual orientations of tRNAs, including conventional R and S. Moreover, the tRNA flexibility allows novel mutual orientations of tRNAs in which tRNA molecules, as in conventional R and S orientations, also form the codon-anticodon duplexes, and the CCA-ends are located adjacently. The R and S orientations do not offer any advantages over the novel orientations. Therefore, besides the conventional R and S orientations, the novel orientations should also be considered, i.e. the interpretations of the available experimental data on the mutual orientations of tRNAs should be reconsidered. All mutual orientations of tRNAs that are compatible with the available experimental data are given.
对现有数据的分析表明，第i个密码子-反密码子双链体中的一个密码子应与第i - 1个双链体的摆动碱基对相互作用。这种双链体间的相互作用应在核糖体延伸循环的全过程中发生，以防止非程序性移码。实验观察到的tRNA的灵活性使得在tRNA的不同相互取向（包括传统的R型和S型）下都能保持双链体间的相互作用。此外，tRNA的灵活性允许tRNA有新的相互取向，在这些取向中，tRNA分子如同在传统的R型和S型取向中一样，也形成密码子-反密码子双链体，且CCA末端相邻定位。R型和S型取向相对于新的取向没有任何优势。因此，除了传统的R型和S型取向之外，还应考虑新的取向，即应重新审视关于tRNA相互取向的现有实验数据的解释。给出了与现有实验数据相符的tRNA的所有相互取向。
