Osterhout G J, Valentine J L, Dick J D
Department of Pathology, Division of Microbiology, Johns Hopkins Hospital, Baltimore, Maryland 21287-7093, USA.
J Clin Microbiol. 1998 Sep;36(9):2618-22. doi: 10.1128/JCM.36.9.2618-2622.1998.
CDC group IVc-2 is a gram-negative, oxidase-positive, nonfermentative bacillus that has been implicated in human infections, including septicemia and peritonitis. Biochemically it most closely resembles Bordetella bronchiseptica and Alcaligenes sp. Results of cellular fatty acid (CFA) and 16S rRNA gene analysis were combined with biochemical data to assist in identification and classification. The predominant CFAs were hexadecanoic acid (16:0), cis-9-hexadecanoic acid (16:1omega7c), cis-11-octadecanoic acid (18:1omega7c), and Delta-cis-9,10-methylenehexadecanoic acid (17:0cyc). Small amounts (2 to 5%) of 3-hydroxytetradecanoic acid (3-OH-14:0), tetradecanoic acid (14:0), 2-hydroxyhexadecanoic acid (2-OH-16:0), and Delta-cis-11,12-methyleneoctadecanoic acid (19:0cyc) were also consistently present. The highest 16S rRNA gene similarity was with Ralstonia eutropha and Ralstonia solanacearum. The CFA and 16S rRNA gene sequence data support the inclusion of CDC group IVc-2 in the recently created genus Ralstonia, which includes R. eutropha, R. pickettii, and R. solanacearum.
疾病控制与预防中心（CDC）IVc-2群是一种革兰氏阴性、氧化酶阳性、不发酵的杆菌，可导致人类感染，包括败血症和腹膜炎。在生化特性上，它与支气管败血博德特氏菌和产碱杆菌最为相似。细胞脂肪酸（CFA）和16S rRNA基因分析结果与生化数据相结合，以协助进行鉴定和分类。主要的细胞脂肪酸为十六烷酸（16:0）、顺-9-十六烷酸（16:1ω7c）、顺-11-十八烷酸（18:1ω7c）和δ-顺-9,10-亚甲基十六烷酸（17:0cyc）。还始终存在少量（2%至5%）的3-羟基十四烷酸（3-OH-14:0）、十四烷酸（14:0）、2-羟基十六烷酸（2-OH-16:0）和δ-顺-11,12-亚甲基十八烷酸（19:0cyc）。16S rRNA基因的最高相似度与真养雷尔氏菌和茄科雷尔氏菌。细胞脂肪酸和16S rRNA基因序列数据支持将CDC IVc-2群归入最近创建的雷尔氏菌属，该属包括真养雷尔氏菌、皮氏雷尔氏菌和茄科雷尔氏菌。
