Moissenet D, Bidet P, Garbarg-Chenon A, Arlet G, Vu-Thien H
Service de Microbiologie, Hôpital d'Enfants Armand-Trousseau, Faculté de Médecine Saint Antoine, Université Paris VI, Paris, France.
J Clin Microbiol. 2001 Jan;39(1):381-4. doi: 10.1128/JCM.39.1.381-384.2001.
Ralstonia paucula (formerly CDC group IV c-2) can cause serious human infections. Confronted in 1995 with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic DNA analysis was poorly discriminatory. In this study, we used PCR-ribotyping and PCR-restriction fragment length polymorphism analysis of the spacer 16S-23S ribosomal DNA (rDNA); both methods were unable to differentiate R. paucula isolates. Eighteen strains belonging to other Ralstonia species (one R. eutropha strain, six R. pickettii strains, three R. solanacearum strains, and eight R. gilardii strains) were also tested by PCR-ribotyping, which failed to distinguish between the four species. The 16S-23S rDNA intergenic spacer of R. paucula contains the tRNA(Ile) and tRNA(Ala) genes, which are identical to genes described for R. pickettii and R. solanacearum.
少动罗尔斯通氏菌（以前的疾病控制和预防中心IV c-2组）可引起严重的人类感染。1995年面对5例医院获得性菌血症病例，我们发现脉冲场凝胶电泳无法区分分离株，随机扩增多态性DNA分析的鉴别能力也很差。在本研究中，我们对间隔区16S-23S核糖体DNA（rDNA）进行了PCR核糖体分型和PCR限制性片段长度多态性分析；两种方法均无法区分少动罗尔斯通氏菌分离株。另外，还对属于其他罗尔斯通氏菌属的18个菌株（1株真养罗尔斯通氏菌、6株皮氏罗尔斯通氏菌、3株青枯罗尔斯通氏菌和8株吉氏罗尔斯通氏菌）进行了PCR核糖体分型检测，结果未能区分这4个菌种。少动罗尔斯通氏菌的16S-23S rDNA基因间隔区含有异亮氨酸tRNA和丙氨酸tRNA基因，这与皮氏罗尔斯通氏菌和青枯罗尔斯通氏菌中描述的基因相同。
