通过连接酶链反应在BACTEC培养物中早期检测结核分枝杆菌。
Early detection of Mycobacterium tuberculosis in BACTEC cultures by ligase chain reaction.
作者信息
Tortoli E, Lavinia F, Simonetti M T
机构信息
Laboratorio di Microbiologia e Virologia, Ospedale di Careggi, 50139 Florence, Italy.
出版信息
J Clin Microbiol. 1998 Sep;36(9):2791-2. doi: 10.1128/JCM.36.9.2791-2792.1998.
Abstract
The LCx Mycobacterium tuberculosis ligase chain reaction system (Abbott Diagnostic Division, Abbott Park, Ill.) was used to detect M. tuberculosis in 150 consecutive BACTEC vials on the day on which a positive growth index (GI) was recorded. By LCx, M. tuberculosis DNA was detected in BACTEC vials on average 2.6 days before the presence of acid-fast bacilli could be confirmed by microscopic examination. A total of 106 of 108 M. tuberculosis isolates were detected without centrifugation from bottles presenting very low GIs (average, 70; median, 33). No false-positive result was obtained from nontuberculous mycobacteria or from isolates with contaminants.
摘要
采用LCx结核分枝杆菌连接酶链反应系统（雅培诊断部，伊利诺伊州雅培公园），在记录到阳性生长指数（GI）当天，对连续150个BACTEC培养瓶中的结核分枝杆菌进行检测。通过LCx检测，在通过显微镜检查确认存在抗酸杆菌之前，平均提前2.6天在BACTEC培养瓶中检测到结核分枝杆菌DNA。在呈现极低生长指数（平均70；中位数33）的培养瓶中，108株结核分枝杆菌分离株中有106株未经过离心即被检测到。非结核分枝杆菌或有污染物的分离株未获得假阳性结果。
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