粟酒裂殖酵母Mcm3p是一种必需的核蛋白，与Nda4p（Mcm5p）紧密结合。
Schizosaccharomyces pombe Mcm3p, an essential nuclear protein, associates tightly with Nda4p (Mcm5p).
作者信息
Sherman D A, Forsburg S L
机构信息
Molecular Biology and Virology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla,CA 92037, USA.
出版信息
Nucleic Acids Res. 1998 Sep 1;26(17):3955-60. doi: 10.1093/nar/26.17.3955.
Abstract
MCM proteins are required for the proper regulation of DNA replication. There are six MCM proteins in all eukaryotes which interact to form a large complex. We report the cloning of fission yeast mcm3 +. mcm3 + is essential and spores carrying a Delta mcm3 disruption arrest with an apparently replicated DNA content. The protein is found constitutively in the nucleus and levels remain constant throughout the cell cycle. Mcm3p binds particularly tightly to Nda4p (Mcm5p), but is loosely associated with the other Schizosaccharomyces pombe MCM proteins. Thus, Mcm3p is a peripheral MCM subunit.
摘要
MCM蛋白是DNA复制正常调控所必需的。所有真核生物中都有六种MCM蛋白，它们相互作用形成一个大型复合体。我们报道了裂殖酵母mcm3 +的克隆。mcm3 +是必需的，携带Δmcm3缺失的孢子会因DNA含量明显复制而停滞。该蛋白在细胞核中持续存在，且在整个细胞周期中水平保持恒定。Mcm3p与Nda4p（Mcm5p）结合特别紧密，但与其他粟酒裂殖酵母MCM蛋白结合松散。因此，Mcm3p是一种外周MCM亚基。
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