非洲爪蟾卵中由MCM/P1蛋白多蛋白复合体对DNA复制的许可。
Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs.
作者信息
Kubota Y, Mimura S, Nishimoto S, Masuda T, Nojima H, Takisawa H
机构信息
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Japan.
出版信息
EMBO J. 1997 Jun 2;16(11):3320-31. doi: 10.1093/emboj/16.11.3320.
Abstract
In eukaryotes, chromosomal DNA is licensed for a single round of replication in each cell cycle. Xenopus MCM3 protein has been implicated in the licensing of replication in egg extract. We have cloned cDNAs encoding five immunologically distinct proteins associated with Xenopus MCM3 as members of the MCM/P1 family. Six Xenopus MCM proteins formed a physical complex in the egg extract, bound to unreplicated chromatin before the formation of nuclei, and apparently displaced from replicated chromatin. The requirement of six XMCM proteins for the replication activity of the egg extract before nuclear formation suggests that their re-association with replicated chromatin at the end of the mitotic cell cycle is a key step for the licensing of replication.
摘要
在真核生物中，染色体DNA在每个细胞周期中仅被许可进行一轮复制。非洲爪蟾MCM3蛋白与卵提取物中的复制许可有关。我们克隆了编码与非洲爪蟾MCM3相关的五种免疫上不同的蛋白质的cDNA，它们作为MCM/P1家族的成员。六种非洲爪蟾MCM蛋白在卵提取物中形成了一个物理复合物，在细胞核形成之前与未复制的染色质结合，并且显然从复制的染色质上被取代。在细胞核形成之前，六种XMCM蛋白对卵提取物复制活性的需求表明，它们在有丝分裂细胞周期结束时与复制的染色质重新结合是复制许可的关键步骤。
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