van Meeteren M E, van Bergeijk J D, van Dijk A P, Tak C J, Meijssen M A, Zijlstra F J
Department of Pharmacology, Erasmus University Rotterdam, The Netherlands.
Mediators Inflamm. 1998;7(3):163-8. doi: 10.1080/09629359891090.
We developed an in vitro organ bath method to measure permeability and contractility simultaneously in murine intestinal segments. To investigate whether permeability and contractility are correlated and influenced by mucosal damage owing to inflammation, BALB/c mice were exposed to a 10% dextran sulphate sodium (DSS) solution for 8 days to induce colitis. The effect of pharmacologically induced smooth muscle relaxation and contraction on permeability was tested in vitro. Regional permeability differences were observed in both control and 10% DSS-treated mice. Distal colon segments were less permeable to 3H-mannitol and 14C-PEG 400 molecules compared with proximal colon and ileum. Intestinal permeability in control vs. 10% DSS mice was not altered, although histologic inflammation score and IFN-gamma pro-inflammatory cytokine levels were significantly increased in proximal and distal colon. IL-1beta levels were enhanced in these proximal and distal segments, but not significantly different from controls. Any effect of pharmacologically induced contractility on intestinal permeability could not be observed. In conclusion, intestinal permeability and contractility are not correlated in this model of experimentally induced colitis in mice. Although simultaneous measurement in a physiological set-up is possible, this method has to be further validated.
我们开发了一种体外器官浴方法，用于同时测量小鼠肠段的通透性和收缩性。为了研究通透性和收缩性是否相关，以及是否受炎症引起的粘膜损伤影响，将BALB/c小鼠暴露于10%硫酸葡聚糖钠（DSS）溶液中8天以诱导结肠炎。在体外测试了药理学诱导的平滑肌舒张和收缩对通透性的影响。在对照小鼠和10% DSS处理的小鼠中均观察到区域通透性差异。与近端结肠和回肠相比，远端结肠段对3H-甘露醇和14C-聚乙二醇400分子的通透性较低。尽管近端和远端结肠的组织学炎症评分和IFN-γ促炎细胞因子水平显著升高，但对照小鼠与10% DSS小鼠的肠道通透性没有改变。这些近端和远端节段中IL-1β水平升高，但与对照无显著差异。未观察到药理学诱导的收缩性对肠道通透性的任何影响。总之，在这种实验性诱导的小鼠结肠炎模型中，肠道通透性和收缩性不相关。虽然在生理环境中进行同步测量是可能的，但这种方法必须进一步验证。
