Tateda K, Matsumoto T, Yamaguchi K
Department of Microbiology, Toho University School of Medicine, Tokyo, Japan.
Mediators Inflamm. 1998;7(3):221-3. doi: 10.1080/09629359891171.
Carrageenan has been widely used as an irritant to evoke inflammation in animals or to selectively deplete macrophages in vivo. Although precise understanding of carrageenan activity is a prerequisite for the experimental use of this polysaccharide, the effects of this agent on host-biological systems are still poorly understood. We investigated the effect of carrageenan on serum concentrations of complement C3 and interleukin (IL)-6, a potent complement-inducing factor. Intraperitoneal administration of carrageenan (4 mg) in mice resulted in an initial fall in serum C3 (70% of control, P < 0.05) between 3 and 6 h, but was followed by a significant rise (180% of the control, P < 0.05) at 24h. Prior to the rise in complement C3, a sharp peak of serum IL-6 was observed at 6h after carrageenan treatment. These results indicate potential of carrageenan to enhance host complement systems, which may be associated with, at least in part, an acute induction of IL-6.
角叉菜胶已被广泛用作刺激物，以在动物体内引发炎症或选择性地消耗巨噬细胞。尽管准确了解角叉菜胶的活性是该多糖实验应用的先决条件，但该试剂对宿主生物系统的影响仍知之甚少。我们研究了角叉菜胶对补体C3血清浓度和白细胞介素（IL）-6（一种有效的补体诱导因子）的影响。给小鼠腹腔注射角叉菜胶（4毫克）导致血清C3在3至6小时之间最初下降（降至对照组的70%，P<0.05），但在24小时时随后显著上升（升至对照组的180%，P<0.05）。在补体C3升高之前，角叉菜胶处理后6小时观察到血清IL-6出现尖峰。这些结果表明角叉菜胶具有增强宿主补体系统的潜力，这可能至少部分与IL-6的急性诱导有关。
