Närkiö-Mäkelä M, Teppo A M, Meri S
Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Finland.
Laryngoscope. 2000 Oct;110(10 Pt 1):1745-9. doi: 10.1097/00005537-200010000-00035.
To analyze whether complement C3a anaphylatoxin, other C3 fragments, interleukin-1beta (IL-1beta), or tumor necrosis factor-alpha (TNF-alpha) contributes to inflammation in chronic otitis media with effusion (OME).
The amount of C3a was measured by enzyme-linked immunoassay. Further breakdown of C3 was analyzed by Western blotting. IL-1beta and TNF-alpha concentrations were measured by radioimmunoassay. Bacteria were analyzed by culture and polymerase chain reaction.
Highly elevated levels of C3a and other C3 cleavage fragments were found in all middle ear effusion (MEE) samples. The mean values (+/- SEM, n = 26) for C3a, IL-1beta, and TNF-alpha were 5,973 +/- 1,124 ng/mL, 1,043 +/- 490 pg/mL, and 79 +/- 14.3 pg/mL, respectively. Comparison to an average C3 level of 555 (+/-108) microg/mL indicated that at least 40.5% +/- 6% of total C3 had become activated within the MEE. C3a concentrations were higher in the group in which the effusion had been present in the middle ear for a prolonged period (> or =4 mo) (P = .04). Children with multiple tube insertions had higher C3 (P = .006) and TNF-alpha (P = .04) concentrations in their MEE samples than those receiving their first tubes. C3 and C3a concentrations in MEE correlated to each other (correlation coefficient [r] = 0.513, P = .0056), as did concentrations of IL-1beta and TNF-alpha (r = 0.7016, P < .0001). No significant correlation was found between complement C3 or C3a levels and IL-1beta, TNF-alpha, or bacterial growth.
Highly elevated levels of C3a in MEE indicate ongoing complement activation, which is stronger than in almost any other disease demonstrated previously. Elevated C3a levels contribute to chemotactic and inflammatory potential in the MEE and correlate with the chronicity of the disease.
分析补体C3a过敏毒素、其他C3片段、白细胞介素-1β（IL-1β）或肿瘤坏死因子-α（TNF-α）是否在分泌性中耳炎（OME）的炎症中起作用。
采用酶联免疫吸附测定法测量C3a的量。通过蛋白质印迹法分析C3的进一步分解情况。采用放射免疫测定法测量IL-1β和TNF-α的浓度。通过培养和聚合酶链反应分析细菌。
在所有中耳积液（MEE）样本中均发现C3a和其他C3裂解片段水平显著升高。C3a、IL-1β和TNF-α的平均值（±标准误，n = 26）分别为5,973±1,124 ng/mL、1,043±490 pg/mL和79±14.3 pg/mL。与平均C3水平555（±108）μg/mL相比，表明MEE中至少40.5%±6%的总C3已被激活。中耳积液持续时间较长（≥4个月）的组中C3a浓度更高（P = 0.04）。多次置管的儿童其MEE样本中的C3（P = 0.006）和TNF-α（P = 0.04）浓度高于首次置管的儿童。MEE中的C3和C3a浓度相互相关（相关系数[r] = 0.513，P = 0.0056），IL-1β和TNF-α的浓度也是如此（r = 0.7016，P < 0.0001）。未发现补体C3或C3a水平与IL-1β、TNF-α或细菌生长之间存在显著相关性。
MEE中C3a水平显著升高表明补体持续激活，这比之前证明的几乎任何其他疾病中的激活都更强。升高的C3a水平有助于MEE中的趋化和炎症潜能，并与疾病的慢性程度相关。
