Frölich K, Klima F, Dedek J
Institute for Zoo Biology and Wildlife Research, Berlin, Germany.
J Wildl Dis. 1998 Jul;34(3):436-42. doi: 10.7589/0090-3558-34.3.436.
Antibodies against rabbit hemorrhagic disease virus (RHDV) from 352 red fox (Vulpes vulpes) sera collected in Germany (Mecklenburg-Vorpommern) in 1993 were tested by a blocking enzyme-linked immunosorbent assay (ELISA) test kit. Ninety samples with positive or suspected results also were analyzed by the hemagglutination inhibition test (HIT). Eighteen serum samples (5%) were positive with the blocking ELISA and eight of these also were positive with HIT. The 18 positive sera also were tested by blocking ELISA for antibodies against European brown hare syndrome virus (EBHSV) and by sandwich ELISA to detect for antibodies against RHDV and EBHSV antigen. Antibodies were not found against EBHSV using the blocking ELISA. With the sandwich ELISA, six samples were positive against RHDV and also against EBHSV, indicating cross-reactivity between determinants of both viruses. However, antibody titers against RHDV were higher than against EBHSV in five samples, and in one animal titers were similar. In addition, two positive samples were investigated by Western blot immunoassay which showed clear positive reactions with the two main peptide bands of EBHSV and RHDV. Comparison of the areas below the peaks of the bands after immunoblotting indicated that there was a stronger reaction with the two main polypeptides of RHDV than with the two main peptides of EBHSV. This is the first report of calicivirus antibodies in free-ranging red foxes. Based on the specificity of the tests, the antibodies detected against caliciviruses may be induced by RHDV. There is a potential link for RHDV between free-ranging rabbits and foxes.
采用阻断酶联免疫吸附测定(ELISA)试剂盒对1993年在德国(梅克伦堡-前波美拉尼亚)采集的352份赤狐(赤狐属)血清中针对兔出血性疾病病毒(RHDV)的抗体进行检测。对90份结果为阳性或疑似阳性的样本也采用血凝抑制试验(HIT)进行分析。18份血清样本(5%)阻断ELISA检测呈阳性,其中8份HIT检测也呈阳性。还采用阻断ELISA对这18份阳性血清检测针对欧洲棕兔综合征病毒(EBHSV)的抗体,并采用夹心ELISA检测针对RHDV和EBHSV抗原的抗体。采用阻断ELISA未发现针对EBHSV的抗体。采用夹心ELISA时,6份样本针对RHDV和EBHSV均呈阳性,表明两种病毒的决定簇之间存在交叉反应。然而,5份样本中针对RHDV的抗体效价高于针对EBHSV的抗体效价,1只动物的两种抗体效价相似。此外,对2份阳性样本进行了蛋白质印迹免疫分析,结果显示与EBHSV和RHDV的两条主要肽带均有明显阳性反应。免疫印迹后条带峰值以下区域的比较表明,与RHDV的两条主要多肽的反应强于与EBHSV的两条主要肽段的反应。这是关于自由放养赤狐中杯状病毒抗体的首次报道。基于检测的特异性,检测到的针对杯状病毒的抗体可能是由RHDV诱导产生的。自由放养的兔子和狐狸之间可能存在RHDV传播的潜在联系。