Koskinen L K, Korpela M M, Lahtela J T, Laippala P J, Pikkarainen P H, Koivula T A
Department of Clinical Chemistry, Tampere University Hospital, Finland.
Clin Chim Acta. 1998 Jul 6;275(1):53-61. doi: 10.1016/s0009-8981(98)00076-x.
The effects of alcohol and aspirin on HbA1c chromatography in the Mono S method were studied in vitro and in vivo. A modified chromatography with enhanced resolution was used, making possible detailed examination of minor interfering peaks included in the routine HbA1c value. Incubation with acetylsalicylic acid increased a hemoglobin fraction separate from HbA1c. In vivo this fraction was elevated by 0.1% of the total hemoglobin during therapeutic aspirin ingestion for one month. In vitro acetaldehyde generated two labile hemoglobin fractions and slightly increased a minor stable fraction which was also elevated in vivo in both alcoholics and heavy drinkers. In relation to the HbA1c concentration, this stable fraction was equal in both alcoholic groups. We conclude that the in vivo effects of both aspirin and alcohol are negligible in routine HbA1c determination. Factors other than acetaldehyde might account for the unexpected HbA1c values in alcoholics.
在体外和体内研究了酒精和阿司匹林对单克隆S法糖化血红蛋白（HbA1c）色谱分析的影响。采用了一种分辨率更高的改良色谱法，使得能够详细检查常规HbA1c值中包含的微小干扰峰。与乙酰水杨酸孵育会增加一种与HbA1c分离的血红蛋白组分。在体内，在进行为期一个月的阿司匹林治疗期间，该组分占总血红蛋白的比例升高了0.1%。在体外，乙醛产生了两个不稳定的血红蛋白组分，并使一个微小的稳定组分略有增加，该组分在酗酒者和大量饮酒者体内也有所升高。就HbA1c浓度而言，两个酒精组中的这个稳定组分是相等的。我们得出结论，在常规HbA1c测定中，阿司匹林和酒精的体内影响可以忽略不计。除乙醛外的其他因素可能导致酗酒者出现意外的HbA1c值。
