酗酒者中乙醛-血红蛋白加合物的研究。
Investigation of an acetaldehyde-hemoglobin adduct in alcoholics.
作者信息
Stockham T L, Blanke R V
机构信息
Department of Pharmacology, Medical College of Virginia, Virginia Commonwealth University, Richmond.
出版信息
Alcohol Clin Exp Res. 1988 Dec;12(6):748-54. doi: 10.1111/j.1530-0277.1988.tb01339.x.
It has been proposed that an ethanol-related adduct is formed with hemoglobin in chronic alcoholics. This proposal is based on the observation that alcoholics were found to have an elevation of minor hemoglobins but normal amounts of glycosylated hemoglobin (HbA1c). Since acetaldehyde is believed to be the primary candidate involved in adduct formation, investigation was focused on this particular compound. This study describes the attempt to isolate and purify an acetaldehyde-hemoglobin adduct in alcoholics by cation-exchange chromatography and m-aminophenylboronic acid affinity chromatography for the purpose of characterization. Using acetaldehyde concentrations of 5 and 10 mM, an acetaldehyde adduct with hemoglobin was formed in vitro, isolated, and characterized. However, a similar adduct could not be isolated in vitro using a pharmacological concentration of 100 microM acetaldehyde nor could this adduct be isolated in human alcoholic subjects.
有人提出，慢性酗酒者体内会形成与乙醇相关的血红蛋白加合物。这一观点基于以下观察结果：酗酒者体内次要血红蛋白水平升高，但糖化血红蛋白（HbA1c）含量正常。由于乙醛被认为是参与加合物形成的主要候选物质，因此研究聚焦于这一特定化合物。本研究描述了通过阳离子交换色谱法和间氨基苯硼酸亲和色谱法，试图从酗酒者体内分离和纯化乙醛 - 血红蛋白加合物以进行表征的过程。使用5 mM和10 mM的乙醛浓度，在体外形成、分离并表征了乙醛与血红蛋白的加合物。然而，使用100 μM乙醛的药理浓度在体外无法分离出类似的加合物，在人类酗酒者体内也无法分离出该加合物。
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