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本文引用的文献

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Three transitions in the RNA polymerase II transcription complex during initiation.起始过程中RNA聚合酶II转录复合物的三个转变。
EMBO J. 1997 Dec 15;16(24):7468-80. doi: 10.1093/emboj/16.24.7468.
2
Recruitment of the putative transcription-repair coupling factor CSB/ERCC6 to RNA polymerase II elongation complexes.将假定的转录修复偶联因子CSB/ERCC6招募至RNA聚合酶II延伸复合物。
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Promoter escape by RNA polymerase II. Formation of an escape-competent transcriptional intermediate is a prerequisite for exit of polymerase from the promoter.RNA聚合酶II的启动子逃逸。形成具备逃逸能力的转录中间体是聚合酶从启动子处离开的前提条件。
J Biol Chem. 1997 Nov 7;272(45):28175-8. doi: 10.1074/jbc.272.45.28175.
4
Genetic analysis of the large subunit of yeast transcription factor IIE reveals two regions with distinct functions.酵母转录因子IIE大亚基的遗传分析揭示了两个具有不同功能的区域。
Mol Cell Biol. 1997 Sep;17(9):5288-98. doi: 10.1128/MCB.17.9.5288.
5
A role for TFIIH in controlling the activity of early RNA polymerase II elongation complexes.TFIIH在控制早期RNA聚合酶II延伸复合物活性中的作用。
Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):9006-10. doi: 10.1073/pnas.94.17.9006.
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The general transcription factors of RNA polymerase II.RNA聚合酶II的通用转录因子。
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Purification of human RNA polymerase II and general transcription factors.人RNA聚合酶II和通用转录因子的纯化。
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Distinct activated and non-activated RNA polymerase II complexes in yeast.酵母中不同的激活态和非激活态RNA聚合酶II复合物
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9
The multiple roles of transcription/repair factor TFIIH.转录/修复因子TFIIH的多重作用。
Trends Biochem Sci. 1996 Sep;21(9):346-50.
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DNA excision repair.DNA切除修复
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启动子近端停滞是由于无法将转录因子IIH招募到转录复合物中所致，是一个受调控的事件。

Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event.

作者信息

Kumar K P, Akoulitchev S, Reinberg D

机构信息

Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, University of Medicine and Dentistry of New Jersey, 663 Hoes Lane, Piscataway, NJ 08854-5635, USA.

出版信息

Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9767-72. doi: 10.1073/pnas.95.17.9767.

DOI:10.1073/pnas.95.17.9767
PMID:9707550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC21411/
Abstract

Promoter-proximal stalling, a general phenomenon observed during the expression of many RNA polymerase II transcribed genes, is dependent on transcription factor IIH (TFIIH). Reactions lacking TFIIH initiated transcription, but the transcription complex encountered a block to elongation proximal to the promoter. The accumulation of promoter-proximal stalled complexes was reduced in the presence of TFIIH and efficient escape from this site also required an activator. Promoter-proximal stalled complexes could not be induced to resume elongation. Our results indicate that effective recruitment of TFIIH into transcription complexes is achieved during formation of the preinitiation complex at the promoter. The studies establish that promoter clearance is a regulated event that requires TFIIH.

摘要

启动子近端停顿是在许多RNA聚合酶II转录基因表达过程中观察到的普遍现象，它依赖于转录因子IIH（TFIIH）。缺乏TFIIH的反应起始了转录，但转录复合物在启动子近端遇到了延伸障碍。在TFIIH存在的情况下，启动子近端停顿复合物的积累减少，并且从该位点有效逃逸也需要激活剂。启动子近端停顿复合物无法被诱导恢复延伸。我们的结果表明，在启动子处预起始复合物形成过程中，TFIIH被有效招募到转录复合物中。这些研究确定启动子清除是一个需要TFIIH的受调控事件。