启动子近端停滞是由于无法将转录因子IIH招募到转录复合物中所致，是一个受调控的事件。
Promoter-proximal stalling results from the inability to recruit transcription factor IIH to the transcription complex and is a regulated event.
作者信息
Kumar K P, Akoulitchev S, Reinberg D
机构信息
Howard Hughes Medical Institute, Division of Nucleic Acid Enzymology, University of Medicine and Dentistry of New Jersey, 663 Hoes Lane, Piscataway, NJ 08854-5635, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Aug 18;95(17):9767-72. doi: 10.1073/pnas.95.17.9767.
Abstract
Promoter-proximal stalling, a general phenomenon observed during the expression of many RNA polymerase II transcribed genes, is dependent on transcription factor IIH (TFIIH). Reactions lacking TFIIH initiated transcription, but the transcription complex encountered a block to elongation proximal to the promoter. The accumulation of promoter-proximal stalled complexes was reduced in the presence of TFIIH and efficient escape from this site also required an activator. Promoter-proximal stalled complexes could not be induced to resume elongation. Our results indicate that effective recruitment of TFIIH into transcription complexes is achieved during formation of the preinitiation complex at the promoter. The studies establish that promoter clearance is a regulated event that requires TFIIH.
摘要
启动子近端停顿是在许多RNA聚合酶II转录基因表达过程中观察到的普遍现象，它依赖于转录因子IIH（TFIIH）。缺乏TFIIH的反应起始了转录，但转录复合物在启动子近端遇到了延伸障碍。在TFIIH存在的情况下，启动子近端停顿复合物的积累减少，并且从该位点有效逃逸也需要激活剂。启动子近端停顿复合物无法被诱导恢复延伸。我们的结果表明，在启动子处预起始复合物形成过程中，TFIIH被有效招募到转录复合物中。这些研究确定启动子清除是一个需要TFIIH的受调控事件。
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