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通过单克隆抗体试验和改良齐-尼氏染色法检测亚历山大不同水源中的隐孢子虫。

Detection of Cryptosporidia in different water sources in Alexandria by monoclonal antibody test and modified Ziehl Neelsen stain.

作者信息

Youssef M Y, Khalifa A M, el Azzouni M Z

机构信息

Department of Parasitology, Faculty of Medicine, University of Alexandria, Egypt.

出版信息

J Egypt Soc Parasitol. 1998 Aug;28(2):487-96.

PMID:9707677
Abstract

Cryptosporidium paruum has become one of the most important contaminant found in drinking water and is associated with high risk of waterborne diseases particularly for the immunocompromised. In the present study, Cryptosporidium was found in uncovered water tanks, nearby the shore in El Noubareya and El Mahmoudeya canals and in one swimming pool. Direct flourescence monoclonal antibody was more sensitive in detecting the organism. Ziehl-Neelsen although less sensitive, yet it has the advantage that it could recognize other protozoa causing diarrhoea as G. lamblia, B hominis and Cyclospora sp.

摘要

微小隐孢子虫已成为饮用水中发现的最重要污染物之一,并且与水源性疾病的高风险相关,特别是对于免疫功能低下者。在本研究中,在露天水箱、努巴雷亚运河和马哈茂迪亚运河岸边附近以及一个游泳池中发现了隐孢子虫。直接荧光单克隆抗体在检测该生物体时更敏感。齐-尼氏染色法虽然敏感性较低,但它具有能够识别其他引起腹泻的原生动物(如蓝氏贾第鞭毛虫、人芽囊原虫和环孢子虫属)的优势。

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