El-Sherbini Gehad T, Mohammad Khairy A
Cairo Curative Organization, Cairo, Egypt.
J Egypt Soc Parasitol. 2006 Aug;36(2 Suppl):49-58.
To demonstrated the zoonotic cryptosporidiosis. A total of 189 farmers and their farm animals (200= 71 buffaloes, 59 cows, 43 sheep and 27 goats were examined. Stool samples Each stool sample was subjected to the direct wet smear method and Sheather's sugar flotation and acid-ether sedimentation techniques and stained with four stains (Modified Ziehl Neelsen, Safranin, methylene blue, Aniline carbol methyl violet and Giemsa). A positive statistically significant correlation was observed between farmers and their farm animals infected with cryptosporidium parvum proving a zoonotic potential for infection (r = 0.819 P <0.01 for cryptosporidiosis. For detection of Cryptosporidium, 100 % sensitivity was obtained when acid ether concentration technique was used and modified Ziehl-Neelsen stain revealed highest sensitivity of 92.1 %.
为证明人兽共患隐孢子虫病。共检查了189名农民及其农场动物（共200只，其中71头水牛、59头奶牛、43只绵羊和27只山羊）。粪便样本 每个粪便样本均采用直接湿涂片法、谢弗氏糖浮选法和酸醚沉淀技术，并使用四种染色剂（改良齐-尼氏染色、番红、亚甲蓝、石炭酸复红甲基紫和吉姆萨染色）进行染色。观察到感染微小隐孢子虫的农民与其农场动物之间存在统计学上显著的正相关，证明了感染的人兽共患潜力（隐孢子虫病的r = 0.819，P <0.01）。对于隐孢子虫的检测，使用酸醚浓缩技术时获得了100%的灵敏度，改良齐-尼氏染色显示出最高灵敏度为92.1%。
