在MDCK细胞中，8-溴环磷酸腺苷（8-Br-cAMP）可刺激顶端内化但非基底外侧内化的蓖麻毒素向高尔基体的转运。
Transport of apically but not basolaterally internalized ricin to the Golgi apparatus is stimulated by 8-Br-cAMP in MDCK cells.
作者信息
Llorente A, van Deurs B, Sandvig K
机构信息
Institute for Cancer Research, The Norwegian Radium Hospital, Oslo, Norway.
出版信息
FEBS Lett. 1998 Jul 17;431(2):200-4. doi: 10.1016/s0014-5793(98)00754-6.
The plant toxin ricin has to be transported to the Golgi apparatus after endocytosis to exert its toxic effect. In this study we show that transport of apically endocytosed ricin to the Golgi apparatus is stimulated by 8-Br-cAMP in polarized MDCK cells. This stimulation is counteracted by the PKA inhibitor H-89. In contrast, there is no increase in the transport to the Golgi apparatus of ricin internalized from the basolateral membrane. These results suggest that protein kinase A selectively regulates endosome to Golgi transport in these cells.
植物毒素蓖麻毒素在胞吞作用后必须被转运至高尔基体才能发挥其毒性作用。在本研究中，我们发现，在极化的MDCK细胞中，8-溴环磷腺苷（8-Br-cAMP）可刺激顶端胞吞的蓖麻毒素向高尔基体的转运。蛋白激酶A（PKA）抑制剂H-89可抵消这种刺激作用。相比之下，从基底外侧膜内化的蓖麻毒素向高尔基体的转运并未增加。这些结果表明，蛋白激酶A在这些细胞中选择性地调节内体到高尔基体的转运。
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