Sandvig K, Grimmer S, Iversen T G, Rodal K, Torgersen M L, Nicoziani P, van Deurs B
Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo.
Int J Med Microbiol. 2000 Oct;290(4-5):415-20. doi: 10.1016/S1438-4221(00)80055-7.
The plant toxin ricin binds to both glycoproteins and glycolipids with terminal galactose, and the toxin will therefore be endocytosed by the different mechanisms operating in a given cell. After endocytosis the toxin is transported to the Golgi apparatus by a process that differs from the Rab9-dependent transport of mannose-6-phosphate receptors. Retrograde toxin transport from the Golgi apparatus to the endoplasmic reticulum (ER) seems to be a requirement for subsequent toxin translocation to the cytosol where the toxin inhibits protein synthesis enzymatically. By using ricin we have characterized different types of endocytosis and the transport steps used by this toxin.
植物毒素蓖麻毒素能与带有末端半乳糖的糖蛋白和糖脂结合，因此该毒素会通过特定细胞中不同的作用机制被内吞。内吞后，毒素通过一个不同于依赖Rab9的甘露糖-6-磷酸受体转运的过程被运输到高尔基体。毒素从高尔基体向内质网的逆行转运似乎是其随后转运至细胞质溶胶（毒素在其中通过酶促作用抑制蛋白质合成）的必要条件。通过使用蓖麻毒素，我们已经确定了不同类型的内吞作用以及该毒素所采用的转运步骤。
