Wayadande A C, Fletcher J
Department of Plant Pathology, Oklahoma State University, Stillwater, Oklahoma, 74078, USA.
J Invertebr Pathol. 1998 Sep;72(2):126-31. doi: 10.1006/jipa.1998.4753.
A continuous cell line of embryonic origin from the leafhopper Circulifer tenellus, CT 1, was established using a protocol modified from thrips cell culture. The line was used to develop an in vitro model to examine the mode of entry of the plant pathogenic mollicute Spiroplasma citri into insect host cells. Confluent monolayers were achieved in 5-6 months using a simple medium developed for maintaining established leafhopper cell lines. The newly established CT 1 line, and that of another leafhopper, Nephotettix cincticeps, were exposed to S. citri and examined by electron microscopy. S. citri was found to cytadhere and to be present in apparent invaginations of the host cell membranes of cell lines of both leafhopper species, supporting the hypothesis that this pathogen enters its insect host via endocytosis.
利用从蓟马细胞培养改良而来的方案，建立了来自叶蝉Circulifer tenellus的胚胎源连续细胞系CT 1。该细胞系用于建立体外模型，以研究植物病原柔膜菌柑橘螺原体进入昆虫宿主细胞的方式。使用为维持已建立的叶蝉细胞系而开发的简单培养基，在5至6个月内获得了汇合的单层细胞。将新建立的CT 1细胞系以及另一种叶蝉黑尾叶蝉的细胞系暴露于柑橘螺原体，并通过电子显微镜进行检查。发现柑橘螺原体可细胞粘附，并存在于两种叶蝉细胞系宿主细胞膜的明显内陷中，支持了这种病原体通过内吞作用进入其昆虫宿主的假说。
