Kamita Shizuo G, Do Zung N, Samra Aman I, Hagler James R, Hammock Bruce D
Department of Entomology, University of California-Davis, 1 Shields Avenue, Davis, CA 95616, USA.
In Vitro Cell Dev Biol Anim. 2005 May-Jun;41(5-6):149-53. doi: 10.1290/0501002.1.
Four continuous cell lines were established from the embryos of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural and ornamental plantings. The cell lines were designated GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, and GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lines were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction- amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.
从玻璃翅叶蝉（Homalodisca coagulata (Say)）的胚胎中建立了四个连续细胞系，玻璃翅叶蝉是葡萄、杏仁、柑橘、夹竹桃及其他农业和观赏作物种植中细菌性病原体的一种具有重要经济意义的昆虫传播媒介。这些细胞系被命名为GWSS-Z10、GWSS-Z15、GWSS-G3和GWSS-LH。GWSS-Z10、GWSS-Z15和GWSS-G3细胞系在添加10%胎牛血清（FBS）的Ex-Cell 401培养基中培养，而GWSS-LH细胞系在添加20% FBS的LH培养基中培养。对这些细胞系的形态、生长、蛋白质组成及其染色体脱氧核糖核酸的聚合酶链反应扩增模式进行了表征。GWSS-Z10、GWSS-Z15、GWSS-G3和GWSS-LH的群体倍增时间分别为46.2、90.9、100.3和60.2小时。这些细胞系可用于研究叶蝉、蚜虫、角蝉及其他相关昆虫的昆虫致病性病毒以及由这些昆虫传播的植物致病性病毒。
