Goodman Cynthia L, Ringbauer Joseph A, Li Yao-Fa, Lincoln Tamra Reall, Stanley David
USDA, Agricultural Research Service, Biological Control of Insects Research Laboratory, 1503 S. Providence Road, Columbia, MO, 65203, USA.
Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences/IPM Center of Hebei Province/ Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Baoding, 07100, China.
In Vitro Cell Dev Biol Anim. 2017 May;53(5):417-420. doi: 10.1007/s11626-017-0134-5. Epub 2017 Feb 15.
The squash bug, Anasa tristis, is a pest of cucurbits that exerts direct damage on crops and is a vector of plant pathogens. We established cell lines from this insect to serve as tools for basic biology, including virology and immunology, as well as applied studies, such as insecticide development programs. We initiated 15 cell cultures, using nine media or combinations of media. The media yielding the best results were a modification of Kimura's medium and a combination of two commercially available cell culture media (EX-CELL 420 and L15). We designated the two cell lines as BCIRL-AtE-CLG11 and BCIRL-AtE-CLG15. From the AtE-CLG15 line, we isolated two sub-lines, A and B. Of these, the most consistently replicating line was AtE-CLG15A. We determined the doubling time of this line (190 h) and its mean cell diameter (14.5 ± 0.7 μm). We characterized the AtE-CLG15A line using DAF-PCR. The BCIRL-AtE-CLG15A cell line is now available for researchers world-wide.
南瓜缘蝽（Anasa tristis）是葫芦科作物的一种害虫，它会对作物造成直接损害，并且是植物病原体的传播媒介。我们从这种昆虫中建立了细胞系，用作基础生物学研究的工具，包括病毒学和免疫学研究，以及应用研究，如杀虫剂开发项目。我们使用九种培养基或培养基组合启动了15种细胞培养。效果最佳的培养基是木村培养基的改良版以及两种市售细胞培养基（EX-CELL 420和L15）的组合。我们将这两个细胞系命名为BCIRL-AtE-CLG11和BCIRL-AtE-CLG15。从AtE-CLG15系中，我们分离出了两个亚系，A和B。其中，最稳定复制的系是AtE-CLG15A。我们测定了该细胞系的倍增时间（190小时）及其平均细胞直径（14.5±0.7μm）。我们使用DAF-PCR对AtE-CLG15A系进行了特征分析。BCIRL-AtE-CLG15A细胞系现可供全球研究人员使用。
