Okamoto H, Tsuruta Y, Miyahara H, Tanaka O, Matsunaga T
Department of Otorhinolaryngology, Yao Municipal Hospital, Osaka.
Nihon Jibiinkoka Gakkai Kaiho. 1998 Jun;101(6):849-55. doi: 10.3950/jibiinkoka.101.6_849.
Pleomorphic adenoma (PA) of the salivary gland has diverse biological behavior in spite of its being a benign tumor. So the nuclear DNA content of 36 PAs was measured by flow cytometry to determine the relationship between proliferative activity and histopathological variable. DNA histograms were evaluated according to the rate of S and G2+ M phase cells (S + G2M%). We assumed that the DNA histogram measured algebraically, S + G2 M% < or = 0%, is the near diploid pattern and calculated its ratio to the near diploid pattern. A statistically significant difference between PA and normal salivary gland was found by the ratio to the near diploid pattern (P < 0.05), which confirmed the usefulness of the ratio to the near diploid pattern for measuring low proliferative activity. The tumors were divided into 3 groups: epitheloid type, intermediate type and myxochondroid type. In the 3 groups no differences were found by S + G2M% and the ratio to the near diploid pattern. The areas of the tumor were divided into 5 groups according to the ratio in the epitheloid region and the myxochondroid region. In the 5 groups, the area which consists partly of epitheloid components and mostly of myxochondroid components had the lowest ratio to the near diploid pattern and the highest S + G2M%, and the area which consists of only myxochondroid components had the highest ratio to the near diploid pattern and the lowest S + G2M%. Between only these two areas a statistically significant difference was found by the ratio to the near diploid pattern (P < 0.05). We considered that the area which consists partly of epitheloid components, and mostly of myxochondroid components has the highest proliferative activity in PA. Neither aneuploid or polyploid cells were found in any tumor, but the S + G2M% is more than 20% in 4 tumors. None of these high S + G2M% tumors except one recurrent tumor had clinical and histopathological features. A differences between PAs of the parotid glands and PAs of the submandibular glands was not found in S + G2M%.
涎腺多形性腺瘤（PA）虽是良性肿瘤，但其生物学行为多样。因此，采用流式细胞术检测36例PA的核DNA含量，以确定增殖活性与组织病理学变量之间的关系。根据S期和G2 + M期细胞率（S + G2M%）评估DNA直方图。我们假定经代数测量，S + G2M%≤0%的DNA直方图为近二倍体模式，并计算其与近二倍体模式的比值。PA与正常涎腺在近二倍体模式比值上存在统计学显著差异（P < 0.05），这证实了近二倍体模式比值在测量低增殖活性方面的有用性。肿瘤分为3组：上皮样型、中间型和黏液软骨样型。3组在S + G2M%及近二倍体模式比值方面未发现差异。根据上皮样区域和黏液软骨样区域的比值将肿瘤区域分为5组。在这5组中，部分由上皮样成分组成且大部分由黏液软骨样成分组成的区域近二倍体模式比值最低，S + G2M%最高；仅由黏液软骨样成分组成的区域近二倍体模式比值最高，S + G2M%最低。仅这两个区域在近二倍体模式比值上存在统计学显著差异（P < 0.05）。我们认为，部分由上皮样成分组成且大部分由黏液软骨样成分组成的区域在PA中增殖活性最高。在任何肿瘤中均未发现非整倍体或多倍体细胞，但有4例肿瘤的S + G2M%超过20%。除1例复发性肿瘤外，这些高S + G2M%的肿瘤均无临床和组织病理学特征。腮腺PA和下颌下腺PA在S + G2M%方面未发现差异。
