Jagetia G C, Aruna R
Department of Radiobiology, Kasturba Medical College, Manipal, India.
Mutat Res. 1998 Jul 8;415(1-2):131-7. doi: 10.1016/s1383-5718(98)00052-7.
The frequency of micronuclei was evaluated in the bone marrow of mice of either sex administered with 0, 0.625, 1.25, 2.5, 5, 10, 20, 40 and 80 mg/kg b.wt of lead nitrate at 12, 24 and 36 h post-treatment. The frequency of micronucleated polychromatic erythrocytes (MPCE) and micronucleated normochromatic erythrocytes (MNCE) increased significantly at 12, 24 and 36 h after treatment with lead nitrate compared to non-drug treated controls. The frequency of micronuclei did not show a dose related increase and the elevation in the frequency of micronuclei was fluctuating type. One important observation which emerged from this study was that the male mice were more sensitive to the induction of micronuclei compared to female mice. This was evidenced by higher frequencies of MPCE in males than females at all the doses for all the post-treatment time periods. The lead nitrate treatment resulted in a spurt in the erythropoiesis as is evidenced by a significant increase in the ratios of polychromatic to normochromatic erythrocytes (P/N ratio) compared to non-drug treated controls at 12, 24 and 36 h post-treatment. The P/N ratio was significantly higher in females than males at 12 and 24 h post-treatment.
在处理后12、24和36小时，对给予0、0.625、1.25、2.5、5、10、20、40和80mg/kg体重硝酸铅的雌雄小鼠骨髓中的微核频率进行了评估。与未用药物处理的对照组相比，在用硝酸铅处理后12、24和36小时，微核多色红细胞（MPCE）和微核正常色红细胞（MNCE）的频率显著增加。微核频率未显示出与剂量相关的增加，且微核频率的升高呈波动型。该研究得出的一个重要观察结果是，与雌性小鼠相比，雄性小鼠对微核诱导更敏感。这一点在处理后所有时间段的所有剂量下，雄性小鼠的MPCE频率均高于雌性小鼠得到了证明。硝酸铅处理导致红细胞生成激增，这一点可从处理后12、24和36小时与未用药物处理的对照组相比，多色红细胞与正常色红细胞的比率（P/N比率）显著增加得到证明。在处理后12和24小时，雌性小鼠的P/N比率显著高于雄性小鼠。
