Chen Z, Sahashi Y, Matsuo K, Asanuma H, Takahashi H, Iwasaki T, Suzuki Y, Aizawa C, Kurata T, Tamura S
Department of Pathology, National Institute of Infectious Diseases, Tokyo, Japan.
Vaccine. 1998 Oct;16(16):1544-9. doi: 10.1016/s0264-410x(98)00043-7.
The ability of plasmid DNA encoding various influenza viral proteins from the A/PR/8/34 (H1N1) virus to protect against influenza was compared in BALB/c mice. The plasmid DNA encoded hemagglutinin (HA), neuraminidase (NA), matrix protein (M1), nucleoprotein (NP) or nonstructural protein (NS1) in a chicken beta-actin-based expression vector (pCAGGS). Each DNA was inoculated twice 3 weeks apart at a dose of 1 microgram per mouse by particle-mediated DNA transfer to the epidermis (gene gun). Seven days after a second immunization, mice were challenged with the homologous virus and the ability of each DNA to protect mice from influenza was evaluated by decreased lung virus titers and increased survival. Mice, given HA- or NA-expressing DNA, induced a high level of specific antibody response and protected well against the challenge virus. On the other hand, mice given M1-, NP-, or NS1-DNA failed to provide protection, although M1- and NP-DNAs did induce detectable antibody responses. These results indicate that both HA- and NA-expressing DNAs for the surface glycoproteins are most protective against influenza from among the various viral protein-expressing DNAs used here.
在BALB/c小鼠中比较了编码来自A/PR/8/34（H1N1）病毒的各种流感病毒蛋白的质粒DNA预防流感的能力。质粒DNA在基于鸡β-肌动蛋白的表达载体（pCAGGS）中编码血凝素（HA）、神经氨酸酶（NA）、基质蛋白（M1）、核蛋白（NP）或非结构蛋白（NS1）。通过粒子介导的DNA转移至表皮（基因枪），以每只小鼠1微克的剂量，将每种DNA每隔3周接种两次。第二次免疫后7天，用同源病毒攻击小鼠，并通过降低肺病毒滴度和提高存活率来评估每种DNA保护小鼠免受流感侵害的能力。给予表达HA或NA的DNA的小鼠诱导了高水平的特异性抗体反应，并对攻击病毒具有良好的保护作用。另一方面，给予M1、NP或NS1 DNA的小鼠未能提供保护，尽管M1和NP DNA确实诱导了可检测到的抗体反应。这些结果表明，在此使用的各种表达病毒蛋白的DNA中，表达表面糖蛋白HA和NA的DNA对流感的保护作用最强。
