Arikawa Y, Enomoto K, Muratsubaki H, Okazaki M
Food Technology Research Institute of Nagano Prefecture, Japan.
FEMS Microbiol Lett. 1998 Aug 1;165(1):111-6. doi: 10.1111/j.1574-6968.1998.tb13134.x.
In Saccharomyces cerevisiae, the cytosolic and promitochondrial isoenzymes of fumarate reductase are encoded by the FRDS and OSM1 genes, respectively. The product of the OSM1 gene is reported to be required for growth in hypertonic medium. Simultaneous disruption of the FRDS and OSM1 genes resulted in the inability of the yeasts to grow anaerobically on glucose as a carbon source, and disruption of the OSM1 gene caused poor growth under anaerobic conditions. However, the disruption of both the FRDS and/or OSM1 genes had no effect on aerobic growth or growth under hypertonic conditions. These results suggest that the fumarate reductase isoenzymes in Saccharomyces cerevisiae are essential for anaerobic growth but not for growth under hypertonic conditions.
在酿酒酵母中，延胡索酸还原酶的胞质同工酶和前线粒体同工酶分别由FRDS和OSM1基因编码。据报道，OSM1基因的产物是在高渗培养基中生长所必需的。同时破坏FRDS和OSM1基因导致酵母无法在以葡萄糖为碳源的厌氧条件下生长，而破坏OSM1基因会导致在厌氧条件下生长不良。然而，破坏FRDS和/或OSM1基因对有氧生长或在高渗条件下的生长没有影响。这些结果表明，酿酒酵母中的延胡索酸还原酶同工酶对于厌氧生长至关重要，但对于高渗条件下的生长并非必需。
