Bunn W J, Heath H E, LeBlanc P A, Sloan G L
Department of Biological Sciences, University of Alabama, Tuscaloosa 35487-0334, USA.
FEMS Microbiol Lett. 1998 Aug 1;165(1):123-7. doi: 10.1111/j.1574-6968.1998.tb13136.x.
Staphylococcus simulans biovar staphylolyticus produces a staphylolytic glycylglycine endopeptidase (lysostaphin) and a micrococcolytic endo-beta-N-acetylglucosaminidase (hexosaminidase) as proenzymes that are proteolytically processed through multiple intermediates to their mature forms by an extracellular sulfhydryl protease. Analysis of protease production by immunoblots using antiserum prepared against purified protease and by renaturing activity gels using gelatin as the substrate has revealed that the lysostaphin-processing protease also is produced as a proenzyme, which appears to be autocatalytically processed. Very little proprotease could be detected in supernatants from cultures of S. simulans biovar staphylolyticus, which suggested that the protein was being processed before it was released to the culture medium. Analysis of wall-associated proteins revealed that processing of proprotease occurred primarily in the cell wall. Furthermore, processing of prolysostaphin and prohexosaminidase also occurred in the cell wall matrix.
模仿葡萄球菌溶葡萄球菌生物变种产生一种溶葡萄球菌糖基甘氨酸内肽酶（溶葡萄球菌素）和一种溶微球菌内切β-N-乙酰葡糖胺糖苷酶（己糖胺酶），它们以前体酶的形式存在，通过一种细胞外巯基蛋白酶经多种中间体进行蛋白水解加工，形成其成熟形式。使用针对纯化蛋白酶制备的抗血清通过免疫印迹以及使用明胶作为底物通过复性活性凝胶对蛋白酶产生进行分析，结果表明溶葡萄球菌素加工蛋白酶也以前体酶的形式产生，它似乎是自动催化加工的。在模仿葡萄球菌溶葡萄球菌生物变种培养物的上清液中几乎检测不到前体蛋白酶，这表明该蛋白质在释放到培养基之前就正在被加工。对与细胞壁相关的蛋白质进行分析发现，前体蛋白酶的加工主要发生在细胞壁中。此外，前溶葡萄球菌素和前己糖胺酶的加工也发生在细胞壁基质中。
