超小免疫金颗粒：免疫细胞化学的重要探针。

Ultrasmall immunogold particles: important probes for immunocytochemistry.

作者信息

Robinson J M, Takizawa T, Vandré D D, Burry R W

机构信息

Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus 43210, USA.

出版信息

Microsc Res Tech. 1998 Jul 1;42(1):13-23. doi: 10.1002/(SICI)1097-0029(19980701)42:1<13::AID-JEMT3>3.0.CO;2-S.

DOI:10.1002/(SICI)1097-0029(19980701)42:1<13::AID-JEMT3>3.0.CO;2-S

PMID:9712159

Abstract

In this article, we review the immunocytochemical literature with respect to a comparison between conventional colloidal gold and ultrasmall gold particles as immunoprobes. We discuss the relative advantages and disadvantages of each of these types of particles for immunocytochemical applications. We present results from our own laboratories, in which we compared these immunoprobes in selected experimental situations. In addition, we discuss our work on the use of a fluorescently labeled ultrasmall immunoprobe for correlative microscopy.

摘要

在本文中，我们回顾了关于传统胶体金和超小金颗粒作为免疫探针的比较的免疫细胞化学文献。我们讨论了每种类型的颗粒在免疫细胞化学应用中的相对优缺点。我们展示了我们自己实验室的结果，其中我们在选定的实验情况下比较了这些免疫探针。此外，我们还讨论了我们在使用荧光标记的超小免疫探针进行相关显微镜检查方面的工作。