Li B, Kanamaru H, Noriki S, Fukuda M, Okada K
Department of Urology, Fukui Medical University, Japan.
Int J Urol. 1998 Jul;5(4):317-23. doi: 10.1111/j.1442-2042.1998.tb00358.x.
This study investigated the relationships between the numeric aberrations of chromosome 17 and p53 expression, the proliferating cell nuclear antigen labeling index (PCNA-LI) and histopathology, to determine their prognostic significance in bladder cancer.
Using in situ hybridization (ISH) with a biotin-labeled chromosome-specific DNA probe, the copy number of pericentromeric sequences in chromosome 17 were detected within interphase nuclei in formalin-fixed paraffin-embedded sections from 59 nonmetastasized transitional cell carcinomas (TCCs) of the urinary bladder. Expression of p53 and PCNA-LI were determined in serial sections by an immunohistochemical method.
The percentage of hyperdiploid cells for chromosome 17 correlated with p53 overexpression (P< 0.002), PCNA-LI (P< 0.002), increasing tumor grade (P< 0.002) and advanced pathologic stage (P< 0.002). The average percentage of hyperdiploid cells was lower in tumors with negative p53 expression than in tumors with p53 overexpression (P< 0.002). Also, more polysomic TCCs were found in muscle-invasive than in superficial cases (P< 0.01 ), and there was a difference in both p53 overexpression or PCNA-LI between disomic and polysomic TCCs (P< 0.01). Patients with chromosome 17 disomic tumors showed less frequent tumor progression than patients with polysomic tumors (P< 0.05). However, chromosome 17 polysomy was an independent prognostic indicator only for patient survival (P< 0.05).
The occurrence and extent of numeric aberrations of chromosome 17 may be associated with the evolution of aggressive growth in TCC and may be a useful indicator for survival.
本研究调查了17号染色体的数值畸变与p53表达、增殖细胞核抗原标记指数（PCNA-LI）以及组织病理学之间的关系，以确定它们在膀胱癌中的预后意义。
使用生物素标记的染色体特异性DNA探针进行原位杂交（ISH），在59例非转移性膀胱移行细胞癌（TCC）的福尔马林固定石蜡包埋切片的间期核内检测17号染色体着丝粒周围序列的拷贝数。通过免疫组织化学方法在连续切片中测定p53和PCNA-LI的表达。
17号染色体超二倍体细胞的百分比与p53过表达（P<0.002）、PCNA-LI（P<0.002）、肿瘤分级增加（P<0.002）和病理分期进展（P<0.002）相关。p53表达阴性的肿瘤中超二倍体细胞的平均百分比低于p53过表达的肿瘤（P<0.002）。此外，肌肉浸润性病例中发现的多体性TCC比浅表病例更多（P<0.01），二体性和多体性TCC之间在p53过表达或PCNA-LI方面均存在差异（P<0.01）。17号染色体二体性肿瘤患者的肿瘤进展频率低于多体性肿瘤患者（P<0.05）。然而，17号染色体多体性仅是患者生存的独立预后指标（P<0.05）。
17号染色体数值畸变的发生和程度可能与TCC侵袭性生长的演变有关，并且可能是生存的有用指标。
