Endow S A, Waligora K W
Department of Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
Science. 1998 Aug 21;281(5380):1200-2. doi: 10.1126/science.281.5380.1200.
The kinesin motor protein family members move along microtubules with characteristic polarity. Chimeric motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain of plus-end-directed kinesin were analyzed. The Ncd stalk and neck reversed kinesin motor polarity, but mutation of the Ncd neck reverted the chimeric motor to plus-end movement. Thus, residues or regions contributing to motor polarity must be present in both the Ncd neck and the kinesin motor core. The neck-motor junction was critical for Ncd minus-end movement; attachment of the neck to the stalk may also play a role.
驱动蛋白运动蛋白家族成员沿微管以特定极性移动。对嵌合马达进行了分析，这些嵌合马达包含负端定向马达Ncd的柄部和颈部，并与正端定向驱动蛋白的马达结构域融合。Ncd的柄部和颈部逆转了驱动蛋白的马达极性，但Ncd颈部的突变使嵌合马达恢复为向正端移动。因此，对马达极性有贡献的残基或区域必定同时存在于Ncd颈部和驱动蛋白马达核心中。颈部与马达的连接处对Ncd向负端移动至关重要；颈部与柄部的连接可能也起作用。
