用携带志贺毒素1编码基因的噬菌体进行体内转导。
In vivo transduction with shiga toxin 1-encoding phage.
作者信息
Acheson D W, Reidl J, Zhang X, Keusch G T, Mekalanos J J, Waldor M K
机构信息
Division of Geographic Medicine and Infectious Diseases and Tupper Research Institute, Tufts University School of Medicine, Boston, Massachusetts, USA.
出版信息
Infect Immun. 1998 Sep;66(9):4496-8. doi: 10.1128/IAI.66.9.4496-4498.1998.
Abstract
To facilitate the study of intestinal transmission of the Shiga toxin 1 (Stx1)-converting phage H-19B, Tn10d-bla mutagenesis of an Escherichia coli H-19B lysogen was undertaken. Two mutants containing insertions in the gene encoding the A subunit of Stx1 were isolated. The resultant ampicillin-resistant E. coli strains lysogenic for these phages produced infectious H-19B particles but not active toxin. These lysogens were capable of transducing an E. coli recipient strain in the murine gastrointestinal tract, thereby demonstrating that lysogens of Shiga toxin-converting phages give rise to infectious virions within the host gastrointestinal tract.
摘要
为便于研究志贺毒素1（Stx1）转换噬菌体H-19B的肠道传播，对大肠杆菌H-19B溶原菌进行了Tn10d-bla诱变。分离出两个在编码Stx1 A亚基的基因中含有插入片段的突变体。这些噬菌体的溶原性产生的氨苄青霉素抗性大肠杆菌菌株产生了具有感染性的H-19B颗粒，但不产生活性毒素。这些溶原菌能够在小鼠胃肠道中转导大肠杆菌受体菌株，从而证明志贺毒素转换噬菌体的溶原菌可在宿主胃肠道内产生具有感染性的病毒粒子。
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本文引用的文献
1
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Infect Immun. 1998 Jan;66(1):394-7. doi: 10.1128/IAI.66.1.394-397.1998.
2
Modulation of horizontal gene transfer in pathogenic bacteria by in vivo signals.体内信号对病原菌水平基因转移的调控
Cell. 1996 Nov 29;87(5):795-8. doi: 10.1016/s0092-8674(00)81986-8.
3
Characterization of Vibrio cholerae bacteriophage K139 and use of a novel mini-transposon to identify a phage-encoded virulence factor.霍乱弧菌噬菌体K139的特性鉴定及利用新型mini转座子鉴定噬菌体编码的毒力因子
Mol Microbiol. 1995 Nov;18(4):685-701. doi: 10.1111/j.1365-2958.1995.mmi_18040685.x.
4
Enterobacter cloacae producing a Shiga-like toxin II-related cytotoxin associated with a case of hemolytic-uremic syndrome.产志贺样毒素II相关细胞毒素的阴沟肠杆菌与一例溶血尿毒综合征病例相关。
J Clin Microbiol. 1996 Feb;34(2):463-5. doi: 10.1128/jcm.34.2.463-465.1996.
5
A role for bacteriophages in the evolution and transfer of bacterial virulence determinants.噬菌体在细菌毒力决定因素的进化和转移中所起的作用。
Mol Microbiol. 1995 Oct;18(2):201-8. doi: 10.1111/j.1365-2958.1995.mmi_18020201.x.
6
Regulation of the Shiga-like toxin II operon in Escherichia coli.大肠杆菌中志贺样毒素II操纵子的调控
Infect Immun. 1996 Feb;64(2):495-502. doi: 10.1128/iai.64.2.495-502.1996.
7
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Infect Immun. 1993 Mar;61(3):1098-104. doi: 10.1128/iai.61.3.1098-1104.1993.
8
Utilization of the mouse large intestine to select an Escherichia coli F-18 DNA sequence that enhances colonizing ability and stimulates synthesis of type 1 fimbriae.利用小鼠大肠筛选增强定殖能力并刺激1型菌毛合成的大肠杆菌F-18 DNA序列。
Infect Immun. 1993 Apr;61(4):1293-300. doi: 10.1128/iai.61.4.1293-1300.1993.
9
Verotoxinogenic Citrobacter freundii associated with severe gastroenteritis and cases of haemolytic uraemic syndrome in a nursery school: green butter as the infection source.与一所幼儿园严重肠胃炎及溶血尿毒综合征病例相关的产志贺毒素弗氏柠檬酸杆菌：绿色黄油作为感染源
Epidemiol Infect. 1995 Jun;114(3):441-50. doi: 10.1017/s0950268800052158.
10
Shiga-like toxin-converting phages from Escherichia coli strains that cause hemorrhagic colitis or infantile diarrhea.来自导致出血性结肠炎或婴儿腹泻的大肠杆菌菌株的志贺样毒素转化噬菌体。
Science. 1984 Nov 9;226(4675):694-6. doi: 10.1126/science.6387911.
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