Khan M A, Wolf D P, Litt M
Biochim Biophys Acta. 1976 Sep 24;444(2):369-73. doi: 10.1016/0304-4165(76)90380-9.
Canine tracheal mucus was dissolved by a number of mucolytic agents, including disulfide bond reducing agents, hydrogen bond breaking agents, and chaotropic ions, and their effect on rheological properties was assessed. Sodium thiocyanate led to 85-100% dissolution with the maximum retention of elasticity. Thiocyanate exposure did not result in demonstrable alterations in the size or shape of the mucus glycoproteins. Sodium thiocyanate is therefore recommended as a suitable dispersing agent for physiochemical studies of glycoprotein secretions.
犬气管黏液可被多种黏液溶解剂溶解，包括二硫键还原剂、氢键破坏剂和离液离子，并评估了它们对流变学性质的影响。硫氰酸钠可导致85%-100%的溶解，同时最大程度保留弹性。暴露于硫氰酸盐不会导致黏液糖蛋白的大小或形状出现明显改变。因此，推荐硫氰酸钠作为糖蛋白分泌物理化研究的合适分散剂。
