Dubey J P
U.S Department of Agriculture, Agricultural Research Service, Livestock and Poultry Sciences Institute, Beltsville, Maryland 20705-2350, USA.
J Parasitol. 1998 Aug;84(4):862-5.
The survival of sporulated Toxoplasma gondii oocysts in water at -10 C to 70 C for various periods was investigated. Infectivity of T. gondii was tested by bioassay in mice. There was no marked loss of infectivity of oocysts stored at 10 C, 15 C, 20 C, and 25 C for 200 days, whereas there was a 100-fold loss of infectivity of oocysts stored at 30 C for 107 days. Oocysts stored at 35 C were infective for 32 days but not 62 days, at 40 C oocysts were infective for 9 days but not 28 days, at 45 C, oocysts were infective for 1 day but not 2 days, at 50 C oocysts were infective for 1 hr but not 2 hr. At 55 C and 60 C oocysts were rendered noninfective in 2 and 1 min, respectively. Oocysts remained infective up to 54 mo at 4 C and there was no loss of infectivity in oocysts stored for 106 days at -5 C and at -10 C and for 13 mo at 0 C.
研究了孢子化的刚地弓形虫卵囊在-10℃至70℃的水中不同时间段的存活情况。通过在小鼠体内进行生物测定来检测弓形虫的感染性。在10℃、15℃、20℃和25℃下储存200天的卵囊，其感染性没有明显损失，而在30℃下储存107天的卵囊，其感染性损失了100倍。在35℃下储存的卵囊32天内具有感染性，但62天时则无；在40℃下，卵囊9天内具有感染性，但28天时则无；在45℃下，卵囊1天内具有感染性，但2天时则无；在50℃下，卵囊1小时内具有感染性，但2小时时则无。在55℃和60℃下，卵囊分别在2分钟和1分钟内失去感染性。在4℃下，卵囊长达54个月仍具有感染性，在-5℃和-10℃下储存106天以及在0℃下储存13个月的卵囊，其感染性没有损失。
