Wexler M, Bogsch E G, Klösgen R B, Palmer T, Robinson C, Berks B C
Centre for Metalloprotein Spectroscopy and Biology, University of East Anglia, Norwich, UK.
FEBS Lett. 1998 Jul 24;431(3):339-42. doi: 10.1016/s0014-5793(98)00790-x.
Preproteins targeted to the Sec-independent protein transport systems of plant thylakoids and of bacteria both have unusual transfer peptides bearing a consensus twin-arginine motif. Possible mechanistic similarity between the two Sec-independent transport pathways was investigated by assessing the ability of bacterial twin-arginine transfer peptides to direct thylakoid import. High efficiency import was observed. This process was demonstrated to occur specifically via the Sec-independent deltapH pathway and to depend on an intact twin-arginine motif on the transfer peptide. These results provide strong evidence for the operation of mechanistically related Sec-independent protein transport pathways in chloroplasts and bacteria.
靶向植物类囊体和细菌的Sec非依赖性蛋白质转运系统的前体蛋白都具有带有共有双精氨酸基序的异常转运肽。通过评估细菌双精氨酸转运肽指导类囊体导入的能力，研究了这两种Sec非依赖性转运途径之间可能的机制相似性。观察到了高效导入。证明该过程具体通过Sec非依赖性ΔpH途径发生，并依赖于转运肽上完整的双精氨酸基序。这些结果为叶绿体和细菌中机制相关的Sec非依赖性蛋白质转运途径的运作提供了有力证据。
